Fluorescence assays of diameter dependent cellular toxicity.

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Fluorescence assays of diameter dependent cellular toxicity. Fluorescence assays of diameter dependent cellular toxicity. (A–C) Merged epifluorescence images of murine fibroblast cells exposed to 108 NW/mL of 36- or 93-nm-diameter AgNWs for 24 h with the fluorescence channels set to the indicated probe molecules and using the microscope objectives indicated. Colored numbers show the integrated probe fluorescence intensity. (D) Summary of intracellular fluorescent probe quantitation. Error bars report 1 SD on triplicate measurements. Sylvia G. Lehmann et al. PNAS doi:10.1073/pnas.1820041116 ©2019 by National Academy of Sciences