Molecular characterization of VAB Molecular characterization of VAB. (A) Schematic representation of VAB structure. Molecular characterization of VAB. (A) Schematic representation of VAB structure. (B) Deduced amino acid sequence of VAB. Underlining indicates the region of the PH domain. (C-E) Subcellular localization of VAN3-sGFP (C) and VAB-eYFP (D), and their merged image (E). (F-I) BiFC analysis of VAN3 and VAB. (F,G) YFP fluorescence (F) and DIC images (G) of protoplasts co-transformed with VAN3-nYFP and VAB-cYFP. (H,I) YFP fluorescence (H) and DIC images (I) of protoplasts co-transformed with VAL3-nYFP and VAB-cYFP. Scale bars: 10 μm in C-E; 5μ m in F-I. Satoshi Naramoto et al. Development 2009;136:1529-1538 © 2009.