Volume 94, Issue 12, Pages (June 2008)

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Volume 94, Issue 12, Pages 4783-4795 (June 2008) Primary Charge Separation in the Photosystem II Core from Synechocystis: A Comparison of Femtosecond Visible/Midinfrared Pump-Probe Spectra of Wild-Type and Two P680 Mutants Mariangela Di Donato, Rachel O. Cohen, Bruce A. Diner, Jacques Breton, Rienk van Grondelle, Marie Louise Groot Biophysical Journal Volume 94, Issue 12, Pages 4783-4795 (June 2008) DOI: 10.1529/biophysj.107.122242 Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 1 Relative positions of PA, PB, and BA, and of the mutated histidine D2-H197. Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 2 Light-induced P680+/P680 FTIR difference spectra from Synechocystis PS2 core complexes from the WT (a) and D2-His197Ala (b) (T=250K; 4cm−1 resolution). Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 3 EADS resulting from global analysis of time-resolved data from Synechocystis sp. PCC 6803 PS2 WT core complexes using a sequential kinetic scheme with increasing lifetimes. Pump-probe data in the mid-IR region between 1560 and 1780cm−1 were obtained upon excitation at 680nm, 100nJ excitation power. Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 4 Comparison between the long-lived component obtained from the global analysis of the vis-pump/mid-IR-probe data and the P680+H− spectrum measured by light-induced FTIR measurements for WT PS2 core complexes from Synechocystis sp. PCC 6803. Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 5 Comparison between the EADS obtained from global analysis of the time-resolved data from Synechocystis sp. PCC 6803 PS2 core WT and D2-His197Gln, and D2-His197Ala mutants. Pump-probe data in the mid-IR region between 1560 and 1780cm−1 were obtained using 100-nJ excitation pulses at 680nm for the WT and D2-His197Gln samples and at 675nm for the D2-His197Ala mutant. Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 6 (A) Five-compartment model employed for the target analysis of the time-resolved data; the rates given in the scheme are those of the WT. (B) Species-associated difference spectra derived from target analysis for PS2 core complexes from WT Synechocystis sp. PCC 6803: (a) CP43; (b) CP47; (c) RP*/RP1; and (d) RP2. Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 7 Comparison between the excited states of CP43 and CP47 obtained by target analysis on WT samples (solid lines) with those measured for the isolated antenna complexes from spinach (dash-dotted lines). Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 8 Comparison between the RC*/RP1 spectrum obtained by target analysis on PS2 core complex samples (solid line) with the sum of the excited-state spectra (PC and BC) and first radical pair spectra obtained for isolated reaction centers from spinach (dash-dotted line). The PC and BC spectra have been obtained from a target analysis of time-resolved data measured for isolated RCs. In the target model, two excited-state compartments were introduced, one from which charge separation can readily occur (PC), and the other of which transfers on a slow timescale to the first (BC) (for discussion, see Groot et al. (8)). Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 9 Comparison between the spectra of the RC*/RP1 compartment (a) on WT PS2 core complexes with those of the D2-His197Gln (b) and D2-His197Ala (c) mutants, obtained by target analysis. Biophysical Journal 2008 94, 4783-4795DOI: (10.1529/biophysj.107.122242) Copyright © 2008 The Biophysical Society Terms and Conditions