Quality Control of Experimental Conditions• (A) Results from fluorescence microscopy suggest that the blank strips (top row) were clear with no biofilm.

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Quality Control of Experimental Conditions• (A) Results from fluorescence microscopy suggest that the blank strips (top row) were clear with no biofilm or C. albicans; the MRS-grown C. albicans (middle row) showed uniform biofilm matrix while the SD-grown C... Quality Control of Experimental Conditions• (A) Results from fluorescence microscopy suggest that the blank strips (top row) were clear with no biofilm or C. albicans; the MRS-grown C. albicans (middle row) showed uniform biofilm matrix while the SD-grown C. albicans (bottom row) showed a dense biofilm with yeast forms.• (B) Mean (wider blue bars) and coefficient of variation (narrow pink bars) of the absorbance optical density from XTT-assay based on the medium (MRS or SD) used for culturing C. albicans and whether the control was used in later analyses for probiotic treatment using the bacteria or the supernatant.• (C) Bland-Altman plot for agreement between the absorbance values obtained from the MRS-grown and SD-grown C. albicans. The limits of agreement (dashed horizontal lines) were at 0.139 and −0.227 and the mean difference was −0.044 indicating slightly lower estimates of OD from the MRS-grown C. albicans. Pittman's p for equal variation in two methods was 0.008. Shweta Ujaoney et al. J Dent Hyg 2014;88:183-189 ©2014 by American Dental Hygienists Association