BACE2 substrates in mouse CSF. BACE2 substrates in mouse CSF. Volcano plot of proteomic analysis of CSF from Bace1−/− and dKO mice (n = 5). For each relatively quantified protein (representing a dot on the plot), the log2-transformed LFQ intensity ratios of dKO/Bace1−/− was plotted against the −log10-transformed t test P-values. Proteins with a t test P-value <0.05 are marked with open red circles, whereas proteins with P-value >0.05 are shown in blue. Labeled in bold black letters are the four previously identified BACE1 substrate candidates. In green are other transmembrane proteins, not previously identified as substrates of BACE1 or BACE2. No corrections for multiple hypothesis testing were applied in this discovery experiment. Iryna Voytyuk et al. LSA 2018;1:e201800026 © 2018 Voytyuk et al.