Isolation and purification of BMSSCs

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Isolation and purification of BMSSCs Isolation and purification of BMSSCs. (A) Flow cytometric analysis of MACS-sorted STRO-1+ marrow cells demonstrated varying levels of STRO-1 expression. Isolation and purification of BMSSCs. (A) Flow cytometric analysis of MACS-sorted STRO-1+ marrow cells demonstrated varying levels of STRO-1 expression. There were approximately three distinct populations: dull (DULL), intermediate (INT) and bright (BRT). (B) Dual-color flow cytometric analysis of VCAM-1 (PE) expression by STRO-1+ (FITC) marrow cells isolated by MACS depicting a discrete population of STRO-1BRIGHT/VCAM-1+ cells (region: R1), approximately 0.02% of the total BMMNC population. (C) The incidence of clonogenic cell colonies (>50 cells) + clusters (>10<50 cells) based on STRO-1BRIGHT/VCAM-1+ expression was determined by limiting dilution analysis of 24 replicates per cell concentration using Poisson distribution analysis (data derived from six independent experiments). Stan Gronthos et al. J Cell Sci 2003;116:1827-1835 © The Company of Biologists Limited 2003