Fig. 1. Expression of the five miRNAs encoded by two miRNA clusters in mouse sperm and oocytes.(A) qPCR analyses of levels of miR-16 (positive control), miR-34b/c and miR-449a/b/c in wild-type (WT) mouse sperm and oocytes. Expression of the five miRNAs encoded by two miRNA clusters in mouse sperm and oocytes.(A) qPCR analyses of levels of miR-16 (positive control), miR-34b/c and miR-449a/b/c in wild-type (WT) mouse sperm and oocytes. Data are presented as mean±SEM (n = 3). (B) qPCR analyses of levels of miR-16 (positive control), miR-34b/c and miR-449a/b/c in wild-type (WT) and miR-34b/c;miR-449 double knockout (miR-dKO) sperm. Data are presented as mean±SEM (n = 3). Shuiqiao Yuan et al. Biology Open 2015;bio.201410959 © 2015. Published by The Company of Biologists Ltd