Miniaturized amplicon library preparation reaction reduces costs while maintaining microbiome readout integrity. Miniaturized amplicon library preparation reaction reduces costs while maintaining microbiome readout integrity. (a) Total reagent and consumable costs for library preparation across three reaction volumes, 25 µl, 5 µl, and 2.5 µl, which includes the setup of the PCR along with library QC and pooling. (b) The breakdown of costs associated with the PCR (red section from 1a) are further expanded. Jeremiah J. Minich et al. mSystems 2018; doi:10.1128/mSystems.00166-18