MK-8628 suppresses T lymphocyte proliferation.

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MK-8628 suppresses T lymphocyte proliferation. MK-8628 suppresses T lymphocyte proliferation. (A) Shown in the heatmap are the absolute gene expression values of the 11 cell cycle related genes, where each horizontal line represents an individual donor. The color gradient represents center scaled Log2 expression values (−2.5 to 2.5). Lymphocytes isolated from healthy human donors were stimulated with anti-CD3/CD28 mAbs in the presence or absence of 200 nM MK-8628 for 24 h (B) Flow cytometry contour plots of Ki-67 expression in anti-CD3/CD28 mAb–stimulated CD4+ and CD8+ T lymphocytes treated with 0.19 μM MK-8628 or untreated controls after 96 h. (C–F) Dose response depicting frequency and geometric mean fluorescent intensity (MFI) of Ki-67 in CD4+ and CD8+ T lymphocytes as shown in (B). Dose response of tritium incorporation in cpm of anti-CD3/CD28 mAb stimulated CD4+ and CD8+ T lymphocytes treated with various concentrations of MK-8628 after 96 h. Data are from one experimental representative of at least three independent experiments and represent triplicate wells (C–H) or represent individual donors from three independent experiments with four donors per experiment (A); small horizontal red lines indicate the mean. *p < 0.05, ***p < 0.001, ****p < 0.0001, one-way ANOVA with posttest analysis (C–H). Peter Georgiev et al. ImmunoHorizons 2019;3:294-305 Copyright © 2019 The Authors