Volume 58, Issue 2, Pages (August 2000)

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Volume 58, Issue 2, Pages 790-799 (August 2000) Effect of vitamin E-bonded membrane on the 8-hydroxy 2′-deoxyguanosine level in leukocyte DNA of hemodialysis patients Der-Cherng Tarng, Tung-Po Huang, Tsung-Yun Liu, Haw-Wen Chen, Yen-Jen Sung, Yau-Huei Wei Kidney International Volume 58, Issue 2, Pages 790-799 (August 2000) DOI: 10.1046/j.1523-1755.2000.00228.x Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 1 Intracellular production of H2O2 (2′,7′dichlorofluorescin; DCF) and O2-·(ethidium bromide; EB) of unstimulated granulocytes. Blood samples were obtained before dialysis and at 15 and 30 minutes after initiation of dialysis from 12 patients in each group using dialyzers equipped with cellulose (□), polymethylmethacrylate (PMMA; ), polysulfone (PS; ), and vitamin E-modified regenerated cellulose (CL-E; ▪) membranes, respectively. Brackets indicate SD. *P < 0.05 vs. predialysis; **P < 0.01 vs. predialysis; †P < 0.05 vs. cellulose membranes. Kidney International 2000 58, 790-799DOI: (10.1046/j.1523-1755.2000.00228.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 2 Increased production of H2O2 (DCF) and O2-·(EB) in granulocytes by PMA stimulation. The difference in the mean fluorescence between stimulated and unstimulated cells was defined as PMA-induced fluorescence increase. Blood samples were obtained before dialysis and at 15 and 30 minutes after initiation of dialysis from 12 patients in each group using dialyzers equipped with the cellulose (□), PMMA (), PS (), and vitamin E-modified regenerated cellulose (CL-E; ▪) membranes, respectively. Brackets indicate SD. *P < 0.05 vs. predialysis; **P < 0.01 vs. predialysis; †P < 0.05 vs. cellulose membranes. Kidney International 2000 58, 790-799DOI: (10.1046/j.1523-1755.2000.00228.x) Copyright © 2000 International Society of Nephrology Terms and Conditions