Pie charts (for all the mice together) and bar graph (for individual mouse) showing relative percentage of T cells and B cells present in the Raman spectroscopy–investigated.

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Pie charts (for all the mice together) and bar graph (for individual mouse) showing relative percentage of T cells and B cells present in the Raman spectroscopy–investigated area and entire fluorescence images of spleen from C57BL/6 mice spleen tissue sections. Pie charts (for all the mice together) and bar graph (for individual mouse) showing relative percentage of T cells and B cells present in the Raman spectroscopy–investigated area and entire fluorescence images of spleen from C57BL/6 mice spleen tissue sections. Pie chart showing ratio of T cells and B cells present in the investigated spleen tissue sections, (A) in sham mice only from the Raman spectroscopic–imaged regions, (B) in LPS-treated mice only from the Raman spectroscopic–imaged regions, (C) in sham mice from entire fluorescence images, and (D) in LPS-treated mice from entire fluorescence images. Bar graph presenting relative percentage of T cells and B cells present in the fluorescence images of individual mouse spleen tissue section, (E) only from the Raman spectroscopic–imaged regions, and (F) from all the fluorescence images. To evaluate changes in the proportion of B cells and T cells in the investigated mice, the ratio of the areas covered with the respective fluorescence marker was calculated. The biological experiments were repeated four times with one mouse per condition. Three tissue slices were investigated per spleen. Mouse of strain type C57BL/6 were investigated using confocal fluorescence microscopy. Anuradha Ramoji et al. ImmunoHorizons 2019;3:45-60 Copyright © 2019 The Authors