LC8 and its binding partners display broad cellular localization.

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LC8 and its binding partners display broad cellular localization. LC8 and its binding partners display broad cellular localization. (A) Live HeLa cells stably expressing LC8-GFP (green) were transiently transfected with the focal adhesion marker TagBFP-paxillin (blue) and the nucleus marker dsRed-tagged histone H2B (red). The top view images shown on the left represent an optical section located next to the coverslip. LC8-GFP is present throughout the cell but forms puncta at the cell cortex. (B) HeLa cells stably expressing LC8-GFP were fixed with PFA and stained for the endogenous α-tubulin (red) and with DAPI (blue) to visualize the DNA. LC8 accumulates at the kinetochores (box 1) and at the spindle poles (box 2). In both (A) and (B), images were acquired from a single cell each, using confocal spinning disc microscopy. (C) Localization information derived from the COMPARTMENTS program demonstrates that LC8 binding partners are localized to all cellular compartments. High confidence localization data were available for 59 of the 73 eukaryotic proteins listed on the LC8Hub database. Nathan Jespersen et al. LSA 2019;2:e201900366 © 2019 Jespersen et al.