Comparison of Matrigel and type-I-collagen matrix architecture.

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Comparison of Matrigel and type-I-collagen matrix architecture. Comparison of Matrigel and type-I-collagen matrix architecture. Matrigel (10 mg/ml, BD Biosciences) and acid-solubilized type-I collagen (3 mg/ml, Koken, Tokyo, Japan) matrices were prepared as described for ultrastructural analysis by scanning electron microscopy (Lizarraga et al., 2009; Steffen et al., 2008). (A) Matrigel, which is commonly used as a model of the BM, forms a dense gel with small pores compared with the dimensions of a cell. (B) By contrast, type-I collagen, which is often used as a model of the interstitial matrix, forms a typical fibrillar meshwork with large pores. Scale bars: 1 μm. Renaud Poincloux et al. J Cell Sci 2009;122:3015-3024 © The Company of Biologists Limited 2009