Arsenic Induces Human Keratinocyte Apoptosis by the FAS/FAS Ligand Pathway, Which Correlates with Alterations in Nuclear Factor-κB and Activator Protein-1.

Slides:

Advertisements

Similar presentations

The Combined Effects of Hematoporphyrin Monomethyl Ether-SDT and Doxorubicin on the Proliferation of QBC939 Cell Lines Lei Liang, Sheng Xie, Lin Jiang,

Advertisements

Crucial Roles of MZF1 and Sp1 in the Transcriptional Regulation of the Peptidylarginine Deiminase Type I Gene (PADI1) in Human Keratinocytes Sijun Dong,

Peroxidated Squalene Induces the Production of Inflammatory Mediators in HaCaT Keratinocytes: A Possible Role in Acne Vulgaris Monica Ottaviani, Theodosis.

Modification of Alternative Splicing of Mcl-1 Pre-mRNA Using Antisense Morpholino Oligonucleotides Induces Apoptosis in Basal Cell Carcinoma Cells Jeng-Jer.

Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor Hiroshi Ishihara, Hiroshi Yoshimoto,

Testosterone promotes apoptotic damage in human renal tubular cells

Histone deacetylase inhibitors increase microRNA-146a expression and enhance negative regulation of interleukin-1β signaling in osteoarthritis fibroblast-like.

Ultraviolet Irradiation Induces Apoptosis in Human Immature, But Not in Skin Mast Cells Sven Guhl, Silke Tapkenhinrichs, Alina Smorodchenko, Andreas Grützkau,

Substance P Enhances the Production of Interferon-induced Protein of 10 kDa by Human Keratinocytes in Synergy with Interferon-γ Naoko Kanda, Shinichi.

PPAR-γ agonist protects podocytes from injury

An Interaction between Arsenic-Induced Epigenetic Modification and Inflammatory Promotion in a Skin Equivalent during Arsenic Carcinogenesis Wei-Ting.

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Elevated expression of CD147 in patients with endometriosis and its role in regulating apoptosis and migration of human endometrial cells Aihong Jin,

Indomethacin Sensitizes TRAIL-Resistant Melanoma Cells to TRAIL-Induced Apoptosis through ROS-Mediated Upregulation of Death Receptor 5 and Downregulation.

Volume 65, Issue 5, Pages (May 2004)

IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway Hye Jung Kim, Tae-Yoon Kim Journal.

All-trans Retinoic Acid Induces Differentiation and Apoptosis of Murine Melanocyte Precursors with Induction of the Microphthalmia-Associated Transcription.

Volume 42, Issue 1, Pages (January 2005)

Involvement of Fas (APO-1/CD-95) during Photodynamic-Therapy-Mediated Apoptosis in Human Epidermoid Carcinoma A431 Cells Nihal Ahmad, Sanjay Gupta, Denise.

Type II Collagen Accumulation in Overlying Dermo-Epidermal Junction of Pilomatricoma Is Mediated by Bone Morphogenetic Protein 2 and 4 Hideki Mieno,

Enhanced Death Ligand-Induced Apoptosis in Cutaneous SCC Cells by Treatment with Diclofenac/Hyaluronic Acid Correlates with Downregulation of c-FLIP

The Selective Protein Kinase C β Inhibitor Enzastaurin Induces Apoptosis in Cutaneous T-Cell Lymphoma Cell Lines through the AKT Pathway Christiane Querfeld,

Selective Induction of Apoptosis in Melanoma Cells by Tyrosinase Promoter-Controlled CD95 Ligand Overexpression Lothar F. Fecker, Christoph C. Geilen,

Toshiyuki Yamamoto, Kiyoshi Nishioka

Bax Activation and Induction of Apoptosis in Human Keratinocytes by the Protein Kinase C δ Catalytic Domain Leonid A. Sitailo, Shalini S. Tibudan, Mitchell.

Ginsenoside F1 Protects Human HaCaT Keratinocytes from Ultraviolet-B-Induced Apoptosis by Maintaining Constant Levels of Bcl-2 Enn Hee Lee, Si Young.

AKT Status Controls Susceptibility of Malignant Keratinocytes to the Early-Activated and UVB-Induced Apoptotic Pathway David Decraene, An Van Laethem,

Inhibitory Effect of β-Thujaplicin on Ultraviolet B-Induced Apoptosis in Mouse Keratinocytes Takako Baba, Hajime Nakano, Katsuto Tamai, Daisuke Sawamura,

Histamine Enhances the Production of Granulocyte-Macrophage Colony-Stimulating Factor via Protein Kinase Cα and Extracellular Signal-Regulated Kinase.

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Shiou-Hwa Jee Journal of Investigative Dermatology

The p53-Stabilizing Compound CP Enhances Ultraviolet-B-Induced Apoptosis in a Human Melanoma Cell Line MMRU Yvonne Luu, Gang Li, Dr Journal of.

PARP Determines the Mode of Cell Death in Skin Fibroblasts, but not Keratinocytes, Exposed to Sulfur Mustard Dana Anderson, Betty Benton, Zhao-Qi Wang,

Role of Matrix Metalloproteinases in HaCaT Keratinocytes Apoptosis Induced by Loxosceles Venom Sphingomyelinase D Danielle Paixão-Cavalcante, Carmen.

All-Trans-Retinoic Acid Induces Interleukin-8 via the Nuclear Factor-κB and p38 Mitogen-Activated Protein Kinase Pathways in Normal Human Keratinocytes

17β-estradiol Inhibits the Production of RANTES in Human Keratinocytes

Evidence of Increased Apoptosis and Reduced Proliferation in Basal Cell Carcinomas Treated with Tazarotene Augusto Orlandi, Luca Bianchi, Antonio Costanzo,

Different Susceptibility of Malignant versus Nonmalignant Human T Cells Toward Ultraviolet A-1 Radiation-Induced Apoptosis Ritsuko Yamauchi, Akimichi.

Role of NF-κB Activity in Apoptotic Response of Keratinocytes Mediated by Interferon-γ, Tumor Necrosis Factor-α, and Tumor-Necrosis-Factor-Related Apoptosis-Inducing.

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

Differential Gene Induction of Human β-Defensins (hBD-1, -2, -3, and -4) in Keratinocytes Is Inhibited by Retinoic Acid Jürgen Harder, Ulf Meyer-Hoffert,

Resistance of Human Melanoma Cells Against the Death Ligand TRAIL Is Reversed by Ultraviolet-B Radiation via Downregulation of FLIP Elke Zeise, Michael.

Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis Hsin-Su Yu, Gwo-Shing Chen

Molecular Responses to Photogenotoxic Stress Induced by the Antibiotic Lomefloxacin in Human Skin Cells: From DNA Damage to Apoptosis Laurent Marrot,

Gang Li, Jason A. Bush, Vincent C. Ho

In Vitro and In Vivo Anti-Melanoma Effects of Ciglitazone

Bcl-2 Reduced and Fas Activated by the Inhibition of Stem Cell Factor/KIT Signaling in Murine Melanocyte Precursors Satoko Kimura, Tamihiro Kawakami,

Activation of Dual Apoptotic Pathways in Human Melanocytes and Protection by Survivin Tong Liu, Diana Biddle, Adrianne N. Hanks, Brook Brouha, Hui Yan,

Volume 62, Issue 4, Pages (October 2002)

Tumor necrosis factor-α and lipopolysaccharide induce apoptotic cell death in bovine glomerular endothelial cells Udo K. Meßmer, Verena A. Briner, Josef.

A p38MAPK/HIF-1 Pathway Initiated by UVB Irradiation Is Required to Induce Noxa and Apoptosis of Human Keratinocytes Kris Nys, An Van Laethem, Carine.

ΔNp63α Promotes Apoptosis of Human Epidermal Keratinocytes

Decreased Phospholipase D (PLD) Activity in Ceramide-Induced Apoptosis of Human Keratinocyte Cell Line HaCaT Yoshihiko Iwasaki-Bessho, Yoshiko Banno,

Dimethylfumarate Specifically Inhibits the Mitogen and Stress-Activated Kinases 1 and 2 (MSK1/2): Possible Role for its Anti-Psoriatic Effect Borbala.

Hyperthermia Induces Endoplasmic Reticulum-Mediated Apoptosis in Melanoma and Non-Melanoma Skin Cancer Cells Yiqun G. Shellman, William R. Howe, Leslie.

1α,25-Dihydroxyvitamin D3 Stimulates Activator Protein 1 DNA-Binding Activity by a Phosphatidylinositol 3-Kinase/Ras/MEK/Extracellular Signal Regulated.

Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Hajime Iizuka

Expression of Opsin Molecule in Cultured Murine Melanocyte

Lawrence M. Pfeffer, Andrzej T. Slominski

STAT5a/PPARγ Pathway Regulates Involucrin Expression in Keratinocyte Differentiation Xiuju Dai, Koji Sayama, Yuji Shirakata, Yasushi Hanakawa, Kenshi.

Myeloid Differentiation Factor 88 Regulates Basal and UV-Induced Expressions of IL-6 and MMP-1 in Human Epidermal Keratinocytes Youngae Lee, Hyunjung.

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Keratinocyte Apoptosis Induced by Ultraviolet B Radiation and CD95 Ligation – Differential Protection through Epidermal Growth Factor Receptor Activation.

CD40 Ligation Alters the Cell Cycle of Differentiating Keratinocytes

Errata Journal of Investigative Dermatology

Hyun Jeong Park, Hee Jung Kim, Jun Young Lee, Baik Kee Cho, Richard L

The Activity of Caspase-1 Is Increased in Lesional Psoriatic Epidermis

Clusterin Gene Expression Mediates Resistance to Apoptotic Cell Death Induced by Heat Shock and Oxidative Stress Isabelle Viard, Philippe Wehrli, Lan.

Presentation transcript:

Arsenic Induces Human Keratinocyte Apoptosis by the FAS/FAS Ligand Pathway, Which Correlates with Alterations in Nuclear Factor-κB and Activator Protein-1 Activity Wei-Ting Liao, Kee-Lung Chang Journal of Investigative Dermatology Volume 122, Issue 1, Pages 125-129 (January 2004) DOI: 10.1046/j.0022-202X.2003.22109.x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Effects of arsenic on keratinocyte viability. Keratinocytes were treated with different concentrations of sodium arsenite and incubated in a CO2 incubator at 37°C for 24 to 72 h. The cell viability was analyzed by XTT assay. Viability of the control group (no arsenic added; PBS only) was taken as 100% (mean±SD; n=12). Journal of Investigative Dermatology 2004 122, 125-129DOI: (10.1046/j.0022-202X.2003.22109.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Arsenic induced apoptosis and enhanced expression of Fas-associated apoptotic proteins. (A) Cultured keratinocytes were exposed to 1, 5, or 10 μM arsenic for 48 h. Cellular DNA was isolated and performed by 2% agarose gel electrophoresis. The 180-bp DNA ladders were observed as a typical feature for apoptosis (M, 100-bp DNA marker; C, control). (B) Fas-associated apoptotic proteins were detected by Western blotting. Total cellular protein was isolated from 48 h arsenic-treated keratinocytes. FADD, caspase-8 (p18, active form), caspase-3 (p20, active form), and PARP antibodies were used. PARP antibody can react with the 112-kDa PARP and its 85-kDa fragment specifically cleaved by activated caspase-3. Journal of Investigative Dermatology 2004 122, 125-129DOI: (10.1046/j.0022-202X.2003.22109.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Fas neutralization and caspase inhibition assay. Arsenic-exposed keratinocytes were treated with anti-Fas-neutralizing antibody, anti-FasL-neutralizing antibody, or zVAD-fmk. After 48 h of treatment, cell viability was measured by XTT assay. Results were analyzed by one-way ANOVA (*p<0.05, treated group vs. nontreated group; #p<0.05, anti-Fas or anti-FasL antibody-treated group vs. zVAD-fmk-treated group; mean±SD; n=12). Journal of Investigative Dermatology 2004 122, 125-129DOI: (10.1046/j.0022-202X.2003.22109.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Arsenic-altered NF-κB and AP-1 activity. Keratinocytes were exposed to 0.1, 1, 5, and 10 μM of arsenic for 48 h. Activity of NF-κB and AP-1 was detected by pNFκB-TA-Luc and pAP1-Luc luciferase activity assay, respectively. The luciferase activity was normalized with renilla luciferase activity (*p<0.05, treated group vs. nontreated group by Student's t test; mean±SD; n=12). Journal of Investigative Dermatology 2004 122, 125-129DOI: (10.1046/j.0022-202X.2003.22109.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions