SYNCRIP mutant generation and initial SYNCRIP mutant LUMIER experiment. SYNCRIP mutant generation and initial SYNCRIP mutant LUMIER experiment. (A) Schematic representation of full-length SYNCRIP mutant generation. The C-terminal tail of SYNCRIP was digested out of the pDONR vector using one internal and one external restriction site (RS1 and RS2). This vector was gel purified and then ligated with individually synthesized DNA fragments to generate full-length, in-frame SYNCRIP mutants. (B) Number of arginine residues in each designated mini-cluster in the C-terminal tail of SYNCRIP Jonathan Woodsmith et al. LSA 2018;1:e201800178 © 2018 Stelzl et al.