The original syn-BNPs (paenimucillin A [1F] and B [1W]) were synthesized according to Minowa and NRPSPredictor2, two algorithms commonly used for prediction.

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The original syn-BNPs (paenimucillin A [1F] and B [1W]) were synthesized according to Minowa and NRPSPredictor2, two algorithms commonly used for prediction of natural product structures from NRPS biosynthetic gene clusters. The original syn-BNPs (paenimucillin A [1F] and B [1W]) were synthesized according to Minowa and NRPSPredictor2, two algorithms commonly used for prediction of natural product structures from NRPS biosynthetic gene clusters. In this study, we systematically explored the influence of the N-terminal substituent because it is known to influence the antibacterial activity of peptides and the bioinformatic algorithms we used were not designed to provide detailed predictions of this substituent. “>” denotes a MIC of >128 μg/ml (the highest concentration tested). Xavier Vila-Farres et al. mSphere 2018; doi:10.1128/mSphere.00528-17