Fig. 5. Coilin mutants display differential U2 processing activity with specificity toward the CU region.Purified proteins were incubated with in vitro.

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Fig. 5. Coilin mutants display differential U2 processing activity with specificity toward the CU region.Purified proteins were incubated with in vitro transcribed U2 snRNA and the reactions analyzed by both qRT-PCR and agarose gel electrophoresis. Coilin mutants display differential U2 processing activity with specificity toward the CU region.Purified proteins were incubated with in vitro transcribed U2 snRNA and the reactions analyzed by both qRT-PCR and agarose gel electrophoresis. (A) Illustration of U2 snRNA pre-processed transcript with relative primer binding locations for the 5′, CU and 3′ regions; relative length of the mature snRNA is denoted. (B) Histogram of qRT-PCR data following incubation of 100 nM U2 RNA transcript with buffer alone, coilin wild type (WT) or coilin S489D at 200 nM protein. (C) Agarose gel (top) with a portion of the same reactions used as a template for qRT-PCR in B and densitometry graph (bottom) of the major U2 RNA band from gel. (D) Histogram of qRT-PCR data following incubation of 100 nM U2 RNA transcript with GST, GST-coilin WT or GST-coilin d121–291 at 300 ng protein. (E) Agarose gel (top) with a portion of the same reactions used as a template for qRT-PCR in D and densitometry graph (bottom) of the major U2 RNA band from gel. *P<0.05 relative to the wild-type protein in each pair. Three independent incubations of protein (or buffer) with U2 snRNA substrate were conducted. These incubations were used for qRT-PCR with three technical repeats per incubation. Hanna J. Broome et al. Biology Open 2013;2:407-415 © 2013. Published by The Company of Biologists Ltd