Fig. 2. Acetylation stiffens primary cilia.

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Sec1p requires Boi1/2p for proper localization.

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The absence of the centrosome changes global microtubule dynamics in a migrating cell. The absence of the centrosome changes global microtubule dynamics.

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Fig. 2. Acetylation stiffens primary cilia. Acetylation stiffens primary cilia. (A) Ciliary bending stiffness calculated for the cells treated with niltubacin (control) and tubacin. Tubacin treatment increased rigidity by 4-fold. Data presented as mean±s.e.m.; *P<0.05, n=5 per group. (B) Immunostaining against acetylated α-tubulin (red) with cilia marked by SSTR3-GFP (green). The strong increase staining indicates increase in acetylation. Scale bar: 10 µm. (C) Protein expression of acetylated α-tubulin and actin was measured with western blot. Consistent actin bands show the same amount of protein was loaded while strong acetylated α-tubulin band with tubacin treatment confirms increased acetylation previously shown with immunocytochemistry. An M. Nguyen et al. Biology Open 2015;bio.014787 © 2015. Published by The Company of Biologists Ltd