Met-misincorporated residues in Flag-VN–AIMP3 and their location in the AIMP3 and Venus structures. Met-misincorporated residues in Flag-VN–AIMP3 and their.

Slides:

Advertisements

Similar presentations

Fig. 6. Epitope mapping of C12G6.

Advertisements

Volume 19, Issue 12, Pages (December 2011)

Volume 26, Issue 2, Pages e4 (February 2018)

Figure 3 ADAM22 mutant proteins do not bind to LGI1

Evaluation of LC3 and ATG13 dynamics in AS-stimulated microglial cells using live-imaging. Evaluation of LC3 and ATG13 dynamics in AS-stimulated microglial.

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Fig. 1. Overview of the nervous system of the adult S. roscoffensis.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

The C-terminal membrane-proximal region of MARCH8 interacts with TfR.

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

PfSec13 does not co-localize with P. falciparum heterochromatin.

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

PfSec13 is a structural homolog of ScSec13·Nup145C.

Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and.

Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL

Fusion of PALB2 to the BRCT repeats of BRCA1 mediates the assembly of DNA damage foci by PALB2 and RAD51 in BRCA1-deficient cells. Fusion of PALB2 to the.

Simulation of cell arrangement patterns with the RO-only model.

Fig. 2. Centrosomal proteins display distinct localizations and radial distances from centriole walls.U2OS cells were fixed and stained with the indicated.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

LIR motif consensus and structural determinants of LIR–ATG8 interactions. LIR motif consensus and structural determinants of LIR–ATG8 interactions. (A)

HEAT repeats as part of large protein complexes.

Readthrough GPx1 Ter–Neptune proteins colocalize with UPF1 under cytochalasin D treatment. 6CFSMEo- cells transfected with plasmids encoding YFP–UPF1 (green),

Overview of HEAT-motif-containing proteins.

The TGFβ large latent complex (LLC).

Src tethers Cas to adhesion complexes.

Distribution of replicating rDNA in the WT during S phase.

USP2a regulates MYC expression through the MDM2–p53 axis.

Example of a putative bridging fiber.

Colocalisation and influence on migration.

Ezrin in its open, activated conformation binds and colocalizes with MISP at the cell cortex. Ezrin in its open, activated conformation binds and colocalizes.

Attenuated STM expression in drn and drnl mutants.

Expression of transgenic IGF-1Ea propeptide in the skin reduces the severity of contact hypersensitivity responses. Expression of transgenic IGF-1Ea propeptide.

MCAK has an extended conformation on microtubules.

SBF-SEM of mitotic spindles.

The SIM domain of RAD51 is required for HR

The actin organization and N-cadherin dynamics in migrating cells.

Subdiffraction-limit imaging of BRCA1 and 53BP1 in IRIF

Effects of Chd1-knockdown on Hmgpi and Klf5 expression

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

The expression of two forms of N-cadherin.

RAD51 interacts with SUMO-1 through its SIM

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

Peroxisome speeds were slower in patient and control cells.

Misfolding mutations impair the BRCA1 nuclear aggregation in yeast and the BRCA1 nuclear localization in human cells. Misfolding mutations impair the BRCA1.

Fig. 3. Fusion proteins subdividing the chick CaV2

Integrins regulate PFC maturation by regulating dap expression.

Depletion of COPI protein inhibits cis to trans cisternal maturation.

Integrins are required to strengthen Notch signaling.

Degradation of the COPI proteins Ret1 and Sec21 by the AID system.

Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.

Fig. 7. Nrf2-dependent enzyme activities in wild-type, Nrf2- and Keap1-deficient tissues.Hepatic (A,C,E) and cortical (B,D,F) enzyme activities of NQO1.

Integrins modulate the Notch pathway by regulating its intracellular trafficking and/or processing. Integrins modulate the Notch pathway by regulating.

EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed cells. EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Sensory neurons grow and downregulate UCHL1 expression levels during postnatal maturation. Sensory neurons grow and downregulate UCHL1 expression levels.

The regulatory domain of HSF1 is involved in the pro-apoptotic response to TNF. (A) Upper panel, functional domains and potential DAPK phosphorylation.

Ub-binding ability of ERα.

Expression of IP3R subtypes in HEK cells.

Characterization of the ERα-UBS.

Fig. 1 Cryo-EM structure of yeast Elp123 showing its active site.

Model of the role of Vav proteins in apical maturation.

Membrane integration of the model constructs depends on protein sequence, not codon usage. Membrane integration of the model constructs depends on protein.

Vps36 interacts with Smo in the absence of Hh

Subnuclear localization of HA-tagged EBNA1 protein.

Histogram of channel events observed in Cx43-TM (gray) and Cx43-WT-expressing cells. Histogram of channel events observed in Cx43-TM (gray) and Cx43-WT-expressing.

DAPK interacts with HSF1 in vivo and in vitro.

Vps36 regulates the accumulation of Smo and Hh signaling activity.

Effect of myosin Vb shRNA on ezrin phosphorylation and microvilli organization in Caco-2 cells. Effect of myosin Vb shRNA on ezrin phosphorylation and.

ASPP2 hydroxylation is detectable in vivo and the amount of hydroxylation depends on FIH-1 abundance. ASPP2 hydroxylation is detectable in vivo and the.

Presentation transcript:

Met-misincorporated residues in Flag-VN–AIMP3 and their location in the AIMP3 and Venus structures. Met-misincorporated residues in Flag-VN–AIMP3 and their location in the AIMP3 and Venus structures. (A,B) The Met-exchanged residues identified by mass spectrometry analysis are depicted in the AIMP3 (pdb2uz8) (A) and the full Venus (pdb3t6h) (B) structures. Residues only identified from HEK293T cells expressing wild-type (WT) MRS (A,B, upper images) and MRS-SD (A,B, middle images) are depicted in cyan and red, respectively. Common residues identified from both cells are represented in orange. The original Met residue in AIMP3 is depicted in green. Mismethionylated residues on AIMP3 in MRS-SD-overexpressing conditions are also represented on the MRS–AIMP3 complex structure (pdb4bl7) (A, lower images). The GST-domain of MRS is shown in light green. Each N-terminus of Venus (VN) and deleted C-terminus of Venus (VC) in the full Venus protein structure is shown in green and red, respectively, for convenience (B, lower panel). Jin Young Lee et al. J Cell Sci 2014;127:4234-4245 © 2014. Published by The Company of Biologists Ltd