The selective progesterone receptor modulator CDB4124 inhibits proliferation and induces apoptosis in uterine leiomyoma cells Xia Luo, Ph.D., Ping Yin,

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Presentation transcript:

The selective progesterone receptor modulator CDB4124 inhibits proliferation and induces apoptosis in uterine leiomyoma cells Xia Luo, Ph.D., Ping Yin, Ph.D., John S. Coon V., M.S., You-Hong Cheng, Ph.D., Ronald D. Wiehle, Ph.D., Serdar E. Bulun, M.D. Fertility and Sterility Volume 93, Issue 8, Pages 2668-2673 (May 2010) DOI: 10.1016/j.fertnstert.2009.11.031 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Effects of CDB4124 on proliferating cell nuclear antigen expression in leiomyoma smooth muscle (LSM) and myometrial smooth muscle (MSM) cells by Western blot analysis. Blots were reprobed with a β-actin antibody as a loading control. Data were presented from one representative subject. Results were reproduced in cells from two other subjects. (B) Effects of CDB4124 on the number of viable LSM and MSM cells, as assessed by 93-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay. Data were reported as the mean ± SE of the mean using cells from three subjects. Each count was performed using cells cultured in triplicate wells. ∗P<.05 versus vehicle treatment. Fertility and Sterility 2010 93, 2668-2673DOI: (10.1016/j.fertnstert.2009.11.031) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Effects of CDB4124 on cleaved polyadenosine 5′-diphosphate-ribose polymerase (PARP) protein expression in leiomyoma smooth muscle (LSM) cells and myometrial smooth muscle (MSM) cells. Cleaved PARP protein expression was determined by Western blotting. Equal loading was confirmed by immunoblotting with a β-actin antibody. Data were presented from one representative subject. Results were reproduced using cells from two other subjects. Fertility and Sterility 2010 93, 2668-2673DOI: (10.1016/j.fertnstert.2009.11.031) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Effects of CDB4124 on Bcl-2 mRNA levels in leiomyoma smooth muscle (LSM) cells and myometrial smooth muscle (MSM) cells. Data were shown as the mean ± SE of the mean from one representative experiment. Each experiment was performed using cells in triplicate wells and repeated in two other subjects. ∗P<.05 versus vehicle treatment. (B) Effects of CDB4124 on Bcl-2 protein levels in LSM and MSM cells. Blots were reprobed with a β-actin antibody as a loading control. Data shown were from one representative experiment and reproduced using cells from a total of three subjects. Fertility and Sterility 2010 93, 2668-2673DOI: (10.1016/j.fertnstert.2009.11.031) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 4 (A) Regulation of CDB4124 on Krüppel-like transcription factor 11 (KLF11) messenger RNA (mRNA) expression in leiomyoma smooth muscle (LSM) cells and myometrial smooth muscle (MSM) cells. Data were shown as the mean ± SE of the mean from one representative experiment. Each experiment was done in triplicate wells and repeated using cells from two other subjects. ∗P<.05 versus vehicle treatment. (B) Regulation of CDB4124 on KLF11 protein levels in LSM and MSM cells. Blots were reprobed with a β-actin antibody as a loading control. Data shown were from one representative experiment and reproduced using cells from a total of three subjects. Fertility and Sterility 2010 93, 2668-2673DOI: (10.1016/j.fertnstert.2009.11.031) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions