Typical HPLC determination of d-Asp by the o-phthaldialdehyde-N-acetyl-l-cysteine (OPA-NAC) method in the frog brain. Typical HPLC determination of d-Asp.

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Typical HPLC determination of d-Asp by the o-phthaldialdehyde-N-acetyl-l-cysteine (OPA-NAC) method in the frog brain. Typical HPLC determination of d-Asp by the o-phthaldialdehyde-N-acetyl-l-cysteine (OPA-NAC) method in the frog brain. The peak in the chromatograms corresponds to d-Asp with a retention time of about 5 min. Broken lines represent the HPLC gradient program. (A) HPLC analysis of control frog brain. (B) HPLC analysis of d-Asp-treated frog brain. (C) HPLC analysis of d-Asp-treated frog brain after incubation with d-Asp oxidase. The marked decrease in the height of the d-Asp elution peak confirms the presence of d-Asp in the original sample. Lavinia Burrone et al. J Exp Biol 2012;215:3559-3565 © 2012.