Lipopolysaccharide promoted proliferation and invasion of endometriotic stromal cells via induction of cyclooxygenase-2 expression Yasuko Takenaka, M.D.,

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Lipopolysaccharide promoted proliferation and invasion of endometriotic stromal cells via induction of cyclooxygenase-2 expression Yasuko Takenaka, M.D., Fuminori Taniguchi, M.D., Hiroko Miyakoda, M.S., Eri Takai, M.D., Naoki Terakawa, M.D., Tasuku Harada, M.D. Fertility and Sterility Volume 93, Issue 1, Pages 325-327 (January 2010) DOI: 10.1016/j.fertnstert.2009.06.042 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Lipopolysaccharide (LPS) stimulation of prostaglandin (PG) E2 production in endometrial (EMSCs, open bars) and endometriotic (ESCs, solid bars) stromal cells. PGE2 production was increased by adding LPS (10 ng/mL). The means and SEMs were calculated from different patients' EMSC (n = 6) and ESC (n = 7) cultures. Within EMSCs (lowercase a and b) or ESCs (uppercase A, B, and C), the bars that do not share a letter are significantly different. Asterisks indicate significant difference between EMSCs and ESCs (P< .05). (B) Pretreated NS-398 (1 μmol/L, 24 hours) negated LPS-induced (10 ng/mL, 12 hours) PGE2 production in ESCs. Concentrations of PGE2 in the supernatants were measured by ELISA. Asterisks indicate significant difference between EMSCs and ESCs (P< .05). (C) Effect of LPS (10 ng/mL, 3 hours) on cyclooxygenase (COX) 2 gene expression in EMSCs (open bars) and ESCs (solid bars); COX-2 expression was determined by real-time reverse-transcription polymerase chain reaction. Asterisk indicates significant difference between EMSCs and ESCs (P< .05). (D, E) Effect of LPS on EMSCs (open bars) and ESCs (solid bars) on (D) proliferation or (E) invasion. NS-398 (1 μmol/L) was added with or without LPS (10 ng/mL). After 24 hours' posttransfection of siRNA, bromodeoxyuridine or invasion assay was performed. In the only LPS addition, siControl was transfected instead of COX-2 siRNA. The means and SEMs were calculated from different patients' EMSCs (n = 4) and ESCs (n = 4). Within EMSCs (lowercase a) or ESCs (uppercase A and B), the bars that do not share a letter are significantly different. Asterisk indicates significant difference between EMSCs and ESCs (P< .05). Fertility and Sterility 2010 93, 325-327DOI: (10.1016/j.fertnstert.2009.06.042) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions