Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased.

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Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. (A) NIH3T3 cells were transfected with caveolin-1-specific siRNA or control siRNA, as described. Expression of caveolin-1, survivin, actin and caveolin-2 was assessed by western blotting. A result representative of three independent experiments is shown. (B) Caveolin-1 and survivin levels were quantified by scanning densitometry. The ratios for caveolin-1 to actin (black bar), caveolin-2 to actin (white bar) and survivin to actin are shown as a percentage of the values obtained in control experiments. # and § comparisons with their respective controls, *P<0.01, #P<0.05). (C) Cell proliferation was measured by the MTS® assay. (D) Tcf/Lef reporter assay. NIH3T3 cells were transfected with caveolin-1-specific siRNA or the control siRNA and grown for 24 hours. Then, cells were additionally transfected with the plasmid pTOP-FLASH. After another 24 hours, luciferase activity was determined and normalized to β-galactosidase activity, as described above. All values are mean ± s.e.m. from either three (B) or two (C,D) independent experiments. Vicente A. Torres et al. J Cell Sci 2006;119:1812-1823 © The Company of Biologists Limited 2006