Fig. 8. Compared mobilities of passenger proteins in G2/M-prophase, metaphase and anaphase.FRAP experiments were performed on HeLa cells stably expressing.

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Fig. 8. Compared mobilities of passenger proteins in G2/M-prophase, metaphase and anaphase.FRAP experiments were performed on HeLa cells stably expressing GFP-INCENP or survivin-GFP or aurora kinase B-GFP. Compared mobilities of passenger proteins in G2/M-prophase, metaphase and anaphase.FRAP experiments were performed on HeLa cells stably expressing GFP-INCENP or survivin-GFP or aurora kinase B-GFP. The bleached zone is indicated by the dotted line. The recovery of fluorescence was registered at the times indicated and expressed as relative intensities. The kinetics of recovery are shown on the right side of the cell images. Time is in seconds. Survivin and aurora recovering are already published (Delacour-Larose et al., 2004; Delacour-Larose et al., 2007) and recalled in the figure for comparison. The localization of passenger proteins during mitosis progression is indicated as well as the timing of efficiency of C4 (from G2/M to prometaphase and at anaphase onset). The proposed variations of aurora kinase B affinity for ATP are visualized with arrows. Ly-Thuy-Tram Le et al. Biology Open 2013;bio.20133079 © 2013. Published by The Company of Biologists Ltd