Involvement of PKCα in downregulation of GLP-1 receptor by high glucose in isolated rat islets. Involvement of PKCα in downregulation of GLP-1 receptor.

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Involvement of PKCα in downregulation of GLP-1 receptor by high glucose in isolated rat islets. Involvement of PKCα in downregulation of GLP-1 receptor by high glucose in isolated rat islets. A: Isolated rat islets infected with Ad-GFP were cultured for 3 days in RPMI medium. Panel shows representative islets 3 days after exposure to control Ad-GFP. As seen in this fluorescent micrograph, many cells in islets are infected with the adenovirus. Representative of five evaluations. B: Isolated rat islets were infected with an adenovirus-expressing PKC isoform (α, β2, δ, ε, or ζ), and 3 days after the infection, GLP-1 mRNA levels were evaluated. C: Isolated rat islets were infected with Ad-GFP–or DN-PKCα–expressing adenovirus, and 3 days after the infection the islets were exposed to 5 or 20 mmol/l glucose for 2 days; then GLP-1 mRNA levels were evaluated. *P < 0.05; **P < 0.01 (n = 4 independent experiments). TBP, TATA-binding protein. Gang Xu et al. Diabetes 2007;56:1551-1558 ©2007 by American Diabetes Association