(A) Relative expression levels of the top most down-regulated genes in LATS2L breast tumors (TCGA-BRCA dataset, see Fig 1) in the panel of breast cancer.

Slides:

Advertisements

Similar presentations

Mandal CC et al Supplementary Fig. S1. Increased expression of CSF-1 in human breast cancer cells. (A) The conditioned media from normal human breast epithelial.

Advertisements

B Supplementary Fig S1. (A) ZR75- and MCF7-PELP1 knockdown cells were generated as described in methods section. Pooled colonies were analyzed for PELP1.

The basal and stress‐induced activity of HSF1 is impaired in human aneuploid cells A, BWestern blot analysis for HSP27, HSP70, HSP90 (the used antibody.

From: MicroRNA let-7 Regulates 3T3-L1 Adipogenesis

Constitutive NF-κB activation by the t(11;18)(q21;q21) product in MALT lymphoma is linked to deregulated ubiquitin ligase activity Honglin Zhou, Ming-Qing.

Overexpression of PAI‐1 reverses the arsenite‐mediated effect on senescence Overexpression of PAI‐1 reverses the arsenite‐mediated effect on senescence.

MiR-128b is a potent glucocorticoid sensitizer in MLL-AF4 acute lymphocytic leukemia cells and exerts cooperative effects with miR-221 by Ai Kotani, Daon.

A novel SHP-1/Grb2–dependent mechanism of negative regulation of cytokine-receptor signaling: contribution of SHP-1 C-terminal tyrosines in cytokine signaling.

Essential role of host translation regulatory factors in the assembly of viral translation/replication complex. Essential role of host translation regulatory.

Volume 2, Issue 4, Pages (April 2008)

Ubiquitination of Drp1 by MARCH‐V.

Pirh2 promotes p73 ubiquitination in vivo.

Volume 18, Issue 2, Pages (August 2010)

(A) ZR75-1 cells were treated with 1 μM 5-aza-2′-deoxycytidine (5-Aza) for 4 d. (A) ZR75-1 cells were treated with 1 μM 5-aza-2′-deoxycytidine (5-Aza)

PPARγ signaling correlates with LATS2 in human and mouse tumors and promotes cell death in a LATS2-dependent manner. PPARγ signaling correlates with LATS2.

Expression and regulation of MD-2s.

LATS2 is a tumor suppressor in a mouse lumB breast cancer model.

Volume 21, Issue 12, Pages (December 2017)

Patient neural progenitors show a neurodevelopmental phenotype.

(A) Schematic representation of the conditional Lats1 locus.

B7-H4 expression correlates with MHC-I expression and improved prognosis in patients with breast cancer. B7-H4 expression correlates with MHC-I expression.

Deletion of Lats1 is phenotypically distinct from Lats2 deletion in PyMT tumors. Deletion of Lats1 is phenotypically distinct from Lats2 deletion in PyMT.

LATS2 promotes death of lumB cells.

Volume 6, Issue 6, Pages (December 2004)

Yubao Wang, Ben B. Li, Jing Li, Thomas M. Roberts, Jean J. Zhao

FOXO3a Is Activated in Response to Hypoxic Stress and Inhibits HIF1-Induced Apoptosis via Regulation of CITED2 Walbert J. Bakker, Isaac S. Harris, Tak.

Volume 13, Issue 6, Pages (June 2013)

(A) Genotyping of the Lats2 and Cre alleles in the PyMT-derived cell lines (see primers location in Fig S2A). (A) Genotyping of the Lats2 and Cre alleles.

Volume 13, Issue 1, Pages (January 2008)

Nucleocapsid Phosphorylation and RNA Helicase DDX1 Recruitment Enables Coronavirus Transition from Discontinuous to Continuous Transcription Chia-Hsin.

HDAC1 positively regulates the abundance of miRNAs.

Down-regulation of LATS1 promotes the formation of tumors enriched in basal-like features. Down-regulation of LATS1 promotes the formation of tumors enriched.

Limited transcriptomic changes upon HOTAIR RNA overexpression in MDA‐MB‐231 breast cancer cells Limited transcriptomic changes upon HOTAIR RNA overexpression.

Control of Sister Chromatid Recombination by Histone H2AX

HDAC11 regulates CCL2 expression by recruiting PU.1.

P300 depletion is lethal in cancer cells harboring loss-of-function mutations in CBP. A, synthetic-lethal effects assessed by colony formation assay. p300.

Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. Effect of expression of constitutively active.

Identification of candidate genes within the 1q32 locus.

Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were.

LATS2-associated gene expression pattern is down-regulated specifically in lumB breast tumors. LATS2-associated gene expression pattern is down-regulated.

Metascape Tripathi et al 2015pathways enrichment analysis (Tripathi et al 2015, of differentially expressed genes (counts > 5, FC.

(A) Schematic representation of the conditional Lats2 locus.

(A) Distribution of LATS2 mRNA expression levels in different breast cancer subtypes (PAM50, METABTIC dataset); ***P-value < 0.001, t test comparing lumB.

KGF enhances AECII proliferation and susceptibility to IAV infection, ex vivo. KGF enhances AECII proliferation and susceptibility to IAV infection, ex.

FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.

Effects of SYCE2 expression on DSB repair.

Chd5 deficiency leads to compromised expression of the repressive histone mark H3K27me3 and up-regulation of ribosomal protein genes. Chd5 deficiency leads.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

CREB1 promotes the induction of endogenous ERα target genes.

Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons. Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons.

Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. Ectopic expression of caveolin-1.

CYP1A1 knockdown increases cell death.

LATS2 augments oxidative phosphorylation.

Volume 26, Issue 12, Pages e4 (March 2019)

Regulation of KSHV Lytic Switch Protein Expression by a Virus-Encoded MicroRNA: An Evolutionary Adaptation that Fine-Tunes Lytic Reactivation Priya Bellare,

Constitutive NF-κB activation by the t(11;18)(q21;q21) product in MALT lymphoma is linked to deregulated ubiquitin ligase activity Honglin Zhou, Ming-Qing.

Overexpression of DDB2 reduces invasive abilities in lungs of aggressive breast tumor cells. Overexpression of DDB2 reduces invasive abilities in lungs.

Specific DNA methylation and expression signatures associated with binding of E2A–PBX1 fusion protein. Specific DNA methylation and expression signatures.

Pirh2 represses p73-dependent transactivation.

Osteoactivin is overexpressed in aggressively bone metastatic 4T1 subpopulations versus weakly bone metastatic breast cancer populations. Osteoactivin.

CCR5 enhances both HDR and SSA DNA repair.

Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter. Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Levels and activity of c-myc are increased in a series of stably transfected DAOY and UW228 medulloblastoma cell lines. Levels and activity of c-myc are.

Volume 26, Issue 11, Pages e6 (March 2019)

MYC expression is correlated with dasatinib sensitivity in cancer cell lines and in vivo. MYC expression is correlated with dasatinib sensitivity in cancer.

Fig. 7. JQ1 represses the enhancer-promoter interaction of BRCA1.

KDM4A levels affect the distribution of translation initiation factors

The tumor-suppressive functions of the human INK4A locus

Fig. 2 hTERT induces expression of heat shock protein genes through HSF1 and interacts with Hsp70-1. hTERT induces expression of heat shock protein genes.

Presentation transcript:

(A) Relative expression levels of the top most down-regulated genes in LATS2L breast tumors (TCGA-BRCA dataset, see Fig 1) in the panel of breast cancer cell lines included in the Cancer Cell Line Encyclopedia. (A) Relative expression levels of the top most down-regulated genes in LATS2L breast tumors (TCGA-BRCA dataset, see Fig 1) in the panel of breast cancer cell lines included in the Cancer Cell Line Encyclopedia. Representative luminal cell lines are marked. (B) Upper panel: schematic representation of the LATS2 genomic locus. Positions of CpG island and methylation probes with differential methylation (P-value < 0.05) between LATS2L and LATS2H lumB tumors are shown. Lower panel: mean methylation of each probe in LATS2L versus LATS2H tumors. Line thickness corresponds to the P-value of the difference in methylation levels of the indicated probe between the two groups. For each probe, Pearson's correlation coefficient between expression and methylation in all lumB tumors is listed. (C) Left panel: ZR75-1 cells were transiently transfected with the indicated expression plasmids and harvested 72 h later. Cell extracts were subjected to Western blot analysis of LATS1, LATS2, and GAPDH as loading control. Right panel: ZR75-1 cells were transduced with MYC-LATS2 expression plasmid or vector control and selected with blasticidin to obtain stable expression. Cell extracts were subjected to Western blot analysis with the indicated antibodies. (D) MDA-MB-468 cells were transfected with the indicated expression plasmids and subjected 48 h later to PI exclusion analysis followed by flow cytometry analysis. “Relative cell death” represents the ratio between the percentages of dead (PI+) cells in the GFP-positive population versus the GFP-negative population. Values within each experiment were normalized to GFP transfected sample; mean ± SEM of two independent experiments. Noa Furth et al. LSA 2018;1:e201800171 © 2018 Furth et al.