Expression of the platelet-activating factor receptor in human spermatozoa: differences in messenger ribonucleic acid content and protein distribution.

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Presentation transcript:

Expression of the platelet-activating factor receptor in human spermatozoa: differences in messenger ribonucleic acid content and protein distribution between normal and abnormal spermatozoa William E Roudebush, Ph.D., Mark D Wild, M.D., Elizabeth H Maguire Fertility and Sterility Volume 73, Issue 5, Pages 967-971 (May 2000) DOI: 10.1016/S0015-0282(00)00485-4

FIGURE 1 Immunofluorescent human spermatozoa. Top: Abnormal platelet-activating factor (PAF) receptor distribution. Bottom: Normal PAF receptor distribution. Roudebush. PAF receptor expression. Fertil Steril 2000. Fertility and Sterility 2000 73, 967-971DOI: (10.1016/S0015-0282(00)00485-4)

FIGURE 2 Illustration of the reverse transcription–polymerase chain reaction MIMIC Construction Kit (Clontech Laboratories, Inc., Palo Alto, CA) technique in a single patient sample: DNA ladder (lane 1), empty (lane 2), positive control — MIMIC (310 base pairs) (lane 3), negative control (lane 4), 5.0 × 10−3 attomoles (lane 5), 2.5 × 10−3 attomoles (lane 6), 1.25 × 10−3 attomoles (lane 7), 6.25 × 10−4attomoles (lane 8), 3.125 × 10−4 attomoles (lane 9), and 1.56 × 10−4 attomoles (lane 10). Roudebush. PAF receptor expression. Fertil Steril 2000. Fertility and Sterility 2000 73, 967-971DOI: (10.1016/S0015-0282(00)00485-4)

FIGURE 3 Platelet-activating factor receptor expression in human spermatozoa. Units are 10−3 attomoles/106 spermatozoa. ∗P<.01. Roudebush. PAF receptor expression. Fertil Steril 2000. Fertility and Sterility 2000 73, 967-971DOI: (10.1016/S0015-0282(00)00485-4)