Phylogenetic tree of K. variicola from humans and animals. Phylogenetic tree of K. variicola from humans and animals. Polymorphisms in the K. variicola strains were called against the At-22 reference genome (black cross). All K. variicola isolates identified were ESBL producers. There is one outlier Klebsiella, KPN1705, which projects beyond the border of the figure to the top left. Strains sequenced for this study are represented by circles, while those previously sequenced by Holt et al. (8) are represented by squares. Strains associated with human invasive infection sites are indicated in red, human colonization is indicated in blue, and animal-associated isolates are indicated in green. S. Wesley Long et al. mSphere 2017; doi:10.1128/mSphereDirect.00290-17