Effect of FGF8 on R-C marker expression.

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Effect of FGF8 on R-C marker expression. Effect of FGF8 on R-C marker expression. A, qRT-PCR analysis of ESC-derived neurospheres for R-C marker expression (n = 3; mean ± SEM; ***p < 0.001; **p < 0.01; *p < 0.05; ANOVA with Dunnett’s test). The concentration (ng/ml) of FGF8 is presented as a superscript. B, Representative immunofluorescent images for R-C markers (scale bar = 50 µm). C, Histogram showing the distribution of the immunofluorescent intensity of SP8 and COUP-TFI measured at the single-cell level. Kent Imaizumi et al. eNeuro 2018;5:ENEURO.0368-17.2018 ©2018 by Society for Neuroscience