Fig. 3. MO-mediated smc3 knockdown results in reduced regenerate length, segment length and cell proliferation. MO-mediated smc3 knockdown results in reduced.

Slides:

Advertisements

Similar presentations

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Advertisements

Activin-βA Signaling Is Required for Zebrafish Fin Regeneration

Effectively regenerating livers transiently accumulate proliferative IGF2BP3-positive cells. Effectively regenerating livers transiently accumulate proliferative.

KIN-3 depletion leads to increased levels of centrosomal ZYG-1

CK2 is required for early cell divisions in C. elegans embryos

Effect of cxcr3.2 mutation on dissemination of local mycobacterial infection within 24 hpi. Effect of cxcr3.2 mutation on dissemination of local mycobacterial.

Fig. 6. crb_C mutant photoreceptor cells exhibit normal morphology.

Fig. 6. Ultrastructural changes in the glycocalyx of N

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Fig. 1. Overview of the nervous system of the adult S. roscoffensis.

mTOR downregulation, but not autophagy, reduces VAP(P58S) aggregation.

Fig. 8. CCA and ChQ treatment induce accumulation of F-actin rings.

Fig. 3. Hts and Dlg are in a complex at the postsynaptic membrane of larval NMJs.(A–B″) PLA with HtsM and Dlg antibodies (green) performed on third instar.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 4. smc3 regulates the expression of cx43 in regenerating fins.

Fig. 2. Proportion of motile objects and track length

Overexpression of cx43 rescues smc3-dependent skeletal phenotypes

Fig. 3. Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3.

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

Fig. 7. Knockdown of Meis1 abolishes CR4. 2-GFP expression

X-ray radiographic images showing structural alterations of the joints that are most commonly affected by OA. (A) Normal and (A′) severely affected joint.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. (A,B)

Fig. 1. Loss of PC following INT depletion

Fig. 1. Morphological and growth characterization of hBMCs and hPDCs

Tracking cell fates of the RE using Tg(fn1b:creERt2).

Fig. 1. Pigmentation and melanophore counts of rainbow trout parr and smolt caudal fins.Pigmentation of (A) parr and (B) smolt. Pigmentation and melanophore.

Fig. 1. γ-Tubulin localizes in close proximity to centriole walls in interphase but within an extended PCM meshwork in mitosis.U2OS cells were fixed and.

Fig. 2. Validating the efficiency of smc3 MOs

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Fig. 6. LR phenotype of plastid translation-defective mutants with/without Spec. LR phenotype of plastid translation-defective mutants with/without Spec.

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 7. Lhx1-RNAi reduces the eye size

Fig. 2. Centrosomal proteins display distinct localizations and radial distances from centriole walls.U2OS cells were fixed and stained with the indicated.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Expression of Wnt3EGFP in the cerebellum of Tg(wnt3:Wnt3EGFP)F3 partially compensates cerebellum growth in MO1-injected Wnt3 morphants. Expression of Wnt3EGFP.

Fig. 6. STK35 KO mice show ovary defects.

Fig. 6. Apical polarity of primitive endoderm cells on surface of embryoid bodies.Embryoid bodies were analyzed by immunofluorescence microscopy for GATA4.

An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic progenitor cells. An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic.

Fig. 8. The morphology of the ventral nerve cord in Ror-Myc overexpressing embryos is normal. The morphology of the ventral nerve cord in Ror-Myc overexpressing.

Reb does not play a significant role in regulating TGF-β signaling

Cellular reaction to intraventricular DMSO injection.

Fig. 1. Polarized F-actin cables in the Xenopus neural plate.

Fig. 2. Immunostaining for ClC-5 in isolated hog gastric mucosa

Crim1 colocalizes with integrin and activates the integrin-FAK-ERK signaling pathway. Crim1 colocalizes with integrin and activates the integrin-FAK-ERK.

Fig. 1. Phenotypic characterisation of primary human tubular epithelial cells and human renal fibroblasts. Phenotypic characterisation of primary human.

Fig. 4. Perinuclear dynein regulators are not required for primary ciliogenesis.RPE cells were transfected with siRNA and either nocodazole-treated, fixed,

Fig. 1. Loss of PC following INT depletion

Sox2 regulates dental epithelial stem cell proliferation and differentiation. Sox2 regulates dental epithelial stem cell proliferation and differentiation.

Tumor location determines response to intravenous FOLFIRINOX chemotherapy. Tumor location determines response to intravenous FOLFIRINOX chemotherapy. (A)

Fig. 3. Enhanced EGFR signaling in Rhbdf2P159L/P159L mice.

Fig. 3. Overview of an epizoic flatworm capturing a single Artemia nauplius.(A) Flatworm (Waminoa sp.) on the oral disc of its coral host (G. fascicularis),

Fig. 2. Non-homogeneous subcellular distribution of Vangl2 along the anteroposterior axis. Non-homogeneous subcellular distribution of Vangl2 along the.

Gα12 is not required for hepatic cystogenosis induced by Pkd1 knockout

Fig. 2. iPSCs produce functional osteoblasts.

Male and female Tg(fli1a:EGFP) blood vessel regeneration.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

Delay of tail resorption in trβ crispants during natural metamorphosis

Global cell proliferation replenishes epidermal cells.

Overexpression of cyp7a1 in the liver ameliorates tumor-induced liver inflammation. Overexpression of cyp7a1 in the liver ameliorates tumor-induced liver.

EB1 localization to the centrosome is mediated by its C-terminal domain. EB1 localization to the centrosome is mediated by its C-terminal domain. Sections.

Fig. 2. Acetylation stiffens primary cilia.

Expression of smc3 in whole-mount and cryosectioned regenerating fins

Fig. 3. Mean force and velocity during jumping

Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Proliferative and morphological defects following TbCALM and TbEpsinR depletion. Proliferative and morphological defects following TbCALM and TbEpsinR.

Presentation transcript:

Fig. 3. MO-mediated smc3 knockdown results in reduced regenerate length, segment length and cell proliferation. MO-mediated smc3 knockdown results in reduced regenerate length, segment length and cell proliferation. (A) Representative images of uninjected (UN), smc3 MO-injected (MO1) and Std-MO injected fins. Total regenerate length was calculated by measuring the distance between the amputation plane (indicated by a solid black line) to the distal end of the third fin ray (black arrows indicate the length measured). (B) Graph shows the significant reduction (indicated by *) of regenerate length in smc3 knockdown fins (for both MO1 and MO2) compared to the Std-MO injected fins using the percent similarity method. (C) Representative images of calcein stained fins of uninjected (UN), smc3 MO injected (MO1) and Std-MO injected fins. Segment length was calculated by measuring the distance between first two joints in the 3rd fin ray (black arrows indicates the length measured). Higher magnification images of segments are shown with joints indicated by white arrowheads. (D) Graph shows that significant reduction (indicated by *) of segment length in smc3 knockdown (for both MO1 and MO2) compared to Std-MO injected fins using the percent similarity method. (E) Representative images of H3P-positive cells in uninjected (UN), smc3 MO injected (MO1) and Std-MO injected fins. Measurements were taken from the distal most 250 μm of the 3rd ray. White brackets mark the defined area and n represents the number of H3P-positive cells in that area. Arrows identify H3P-positive cells. (F) Graph shows the significant reduction (indicated by *) in the number of H3P-positive cells in smc3 knockdown (for both MO1 and MO2) compared to Std-MO injected fins using the percent similarity method. For each experiment n=8 fins per trial and three independent trials were performed. *P<0.05, two tailed unpaired Student's t-test. Data are mean±s.e.m. Scale bars: 50 µm in A; 100 µm in E. Rajeswari Banerji et al. Biology Open 2017;6:1802-1813 © 2017. Published by The Company of Biologists Ltd