MR1T clones with distinct TCR usage display differential recognition of discrete activating ligands. MR1T clones with distinct TCR usage display differential.

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MR1T clones with distinct TCR usage display differential recognition of discrete activating ligands. MR1T clones with distinct TCR usage display differential recognition of discrete activating ligands. (A) MR1T clones using the TRAV1-2+ or TRAV1-2− α-chain, or an HLA-B45–restricted T cell clone (D466 A10), responses to DC incubated with 100 μM PLI, PLIII, RL-6,7-diMe, or RL-6-Me-7-OH. M. smegmatis (Msm) for MR1T clones and the CFP102–9 peptide (Pep) for the HLA-B45 clone were used as positive controls. NL indicates the no ligand control condition. (B) hpMR1 loaded with PLI was generated and used to stain the D481C7 and D426G11 clones to demonstrate that distinct TCR-diverse MR1T clone responses to PLI correspond to tetramer staining. Gray histograms are MR1/PLI tetramer staining of a control HLA-A2–restricted CD8+ T cell clone. Results are representative of three independent experiments. Error bars represent means and SD from technical replicates. Melanie J. Harriff et al. Sci. Immunol. 2018;3:eaao2556 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).