Exercise Project Matrix

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Exercise Project Matrix Collaboration with Dr. Sharkey Exercise Project Matrix B B6 strain D D2 strain M male F female E exercise C control Key: B6 D2 M F M F C E C E E 9 9 9 9 9 9 9 9 72 mice total (72 brain RNA samples + 48 bone RNA samples)

Brain RNA QC – quality (260/280) & quantity (ug) April 2005 Brain Dissections Total = 23 samples Sample # Sample type 260/280 [ ] ug/ul ul used for spec ul used for gel ul left from 50ul ~ ug 1 BME 1.8 0.11 10 9 31 3 2 0.12 4 0.13 8 32 BMC 0.10 30 5 6 1.9 0.09 11 29 7 BFE 0.14 33 0.07 14 26 15 BFC 13 DME 12 28 16 DMC 1.7 17 18 19 DFE 20 21 22 DFC 25 23 24 0.1 B B6 strain D D2 strain M male F female E exercise C control Key:

Bone RNA QC – quality (260/280) & quantity (ug) April 2005 Bone Dissections Total = 16 samples Sample # Sample type 260/280 [ ] ug/ul ul used for spec ul used for gel ul left from 50ul ~ ug 1 BME 1.8 0.0904 10 11 29 3 1.7 0.0264 40 4 BMC 0.0848 12 28 2 6 0.0288 7 BFE 0.12 8 32 9 1.6 0.0308 BFC 0.0364 0.042 13 DME 0.0332 15 0.0188 16 DMC 0.068 17 0.0736 19 DFE 1.5 0.0424 21 0.0552 22 DFC 0.058 25 24 1.3 0.0208 B B6 strain D D2 strain M male F female E exercise C control Key:

Bone & Brain RNA QC – quality Bone Samples Brain Samples

Feedback Further RNA QC (bone samples gel & bioanalyzer) Pool samples? Replicates? (# of chips & RT-PCR)