Tyrosine phosphorylation of proteins in podocyte lysates following exposure to healthy or lupus nephritis plasma. Tyrosine phosphorylation of proteins.

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Tyrosine phosphorylation of proteins in podocyte lysates following exposure to healthy or lupus nephritis plasma. Tyrosine phosphorylation of proteins in podocyte lysates following exposure to healthy or lupus nephritis plasma. Human podocytes were incubated with heat-inactivated (HI) plasma from five lupus nephritis (LN) patients and five healthy donors. Cells were lysed following 24 (i) or 48 (ii) h exposure, and protein tyrosine phosphorylation assessed by western blot. This demonstrated globally reduced tyrosine phosphorylation at 24 h in the lupus nephritis plasma exposed-cells, which had returned to normal levels by 48 h. Representative western blots (A) showing tyrosine phosphorylation of podocyte proteins following exposure to plasma from LN patients or from normal controls (NCs). Actin was used to check equal loading of protein. Results were quantified using densitometry and the median and IQR from 5 NCs and 5 patients are shown (B). Data from the two groups were compared using the Mann–Whitney test for significance. Jessica J Manson et al. Lupus Sci Med 2014;1:e000013 ©2014 by Lupus Foundation of America