The vps13 mutants are hypersensitive to SDS but cell wall integrity is not compromised. The vps13 mutants are hypersensitive to SDS but cell wall integrity.

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The vps13 mutants are hypersensitive to SDS but cell wall integrity is not compromised. The vps13 mutants are hypersensitive to SDS but cell wall integrity is not compromised. (A) vps13 mutants are hypersensitive to SDS. (B) Mutants defective in endocytosis are hypersensitive to SDS. (C) Mutants defective in Golgi transport or endocytic sorting are hypersensitive to SDS. (D) Slt2 is hyperphosphorylated in vps13 cells following SDS stress. Overnight yeast cultures were shifted to fresh YPD or YPD+0.03% SDS and incubated for 3 h. Western blot was performed and analysed by densitometry. The phosphorylation levels of Slt2 in relation to Pgk1 were compared by one-way ANOVA followed by Tukey's multiple comparison test (n=3); **P<0.01. Error bars indicate s.d. Piotr Soczewka et al. Dis. Model. Mech. 2019;12:dmm036830 © 2019. Published by The Company of Biologists Ltd