Peli1 levels are reduced in bid−/− microglia upon LPS stimulation. Peli1 levels are reduced in bid−/− microglia upon LPS stimulation. A, B, Peli1 levels were analyzed by Western blot analysis in wild-type and bid−/− microglia upon acute LPS stimulation (B; n = 3 cultures from 3 separate platings; three-way ANOVA, Tukey’s multiple-comparison post hoc test). Cells were stimulated with LPS (100 ng/ml) in serum-free media for the relevant treatment time points, lysed in RIPA buffer and prepared for Western blot analysis. Sinéad Kinsella et al. eneuro 2016;3:ENEURO.0099-15.2016 ©2016 by Society for Neuroscience