CHL cell lines induce a suppressive Treg phenotype in cocultured CD4+ T cells. cHL cell lines induce a suppressive Treg phenotype in cocultured CD4+ T.

Slides:

Advertisements

Similar presentations

c-Myc expression in response to pre-TCR stimulation.

Advertisements

Foxp3 induction dependent on cell cycle progression in vitro.

Melanoma donor (MD) NK cells are functionally impaired/exhausted.

PLAUhigh Tregs exhibit higher potential in suppressing effector T cells and express higher levels of Treg‐important genes, but they express lower levels.

A B C D CD4+ CD8+ Control Isotype Ab Anti-TNFa Ab Control Isotype Ab

by Masih Ostad, Margareta Andersson, Astrid Gruber, and Anne Sundblad

Induction and role of regulatory CD4+CD25+ T cells in tolerance to the transgene product following hepatic in vivo gene transfer by Ou Cao, Eric Dobrzynski,

Ping Zhang, Jieying Wu, Divino Deoliveira, Nelson J. Chao, Benny J

by Norman Nausch, Ioanna E

by Daniel L. Barber, Katrin D. Mayer-Barber, Lis R. V

CD90+ Human Dermal Stromal Cells Are Potent Inducers of FoxP3+ Regulatory T Cells Karin Pfisterer, Karoline M. Lipnik, Erhard Hofer, Adelheid Elbe-Bürger

Expansion of FOXP3high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients by Devi.

Tumor-promoting immune-suppressive myeloid-derived suppressor cells in the multiple myeloma microenvironment in humans by Güllü Topal Görgün, Gregory Whitehill,

The histone methyltransferase Ezh2 is a crucial epigenetic regulator of allogeneic T-cell responses mediating graft-versus-host disease by Shan He, Fang.

Functional human regulatory T cells fail to control autoimmune inflammation due to PKB/c-akt hyperactivation in effector cells by Ellen J. Wehrens, Gerdien.

Induction and maintenance of allergen-specific FOXP3+ Treg cells in human tonsils as potential first-line organs of oral tolerance Oscar Palomares, PhD,

Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses by Cagan Gurer, Till.

Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper by Haifa Hamdi, Véronique.

IL-21 inhibits T cell IL-2 production and impairs Treg homeostasis

Characterization of Interleukin-17–Producing Regulatory T Cells in Inflamed Intestinal Mucosa From Patients With Inflammatory Bowel Diseases Zaruhi Hovhannisyan,

Suppressive effect of CD39+CD73+ melanoma cells on T-cell proliferation, and reversion of this effect via treatment with the CD39-blocking antibody, CD39.

A, RT-qPCR of ROR1 mRNA expression in B-CLL cells and a panel of human and rhesus macaque tissues. A, RT-qPCR of ROR1 mRNA expression in B-CLL cells and.

Gr-1+ MDSC in tumor-bearing mice produce IL-6.

Volume 27, Issue 2, Pages (August 2007)

DCs induce the proliferation of autologous T cells.

Dysregulation of proinflammatory versus anti-inflammatory human TH17 cell functionalities in the autoinflammatory Schnitzler syndrome Rebecca Noster,

A Two-Step Process for Thymic Regulatory T Cell Development

Volume 28, Issue 6, Pages (June 2008)

Bet v 1–specific T-cell receptor/forkhead box protein 3 transgenic T cells suppress Bet v 1–specific T-cell effector function in an activation-dependent.

Volume 30, Issue 6, Pages (June 2009)

Volume 19, Issue 3, Pages (September 2003)

by Derek Hoi-Hang Ho, and Roger Hoi-Fung Wong

2aG4 directly induces monocytic MDSCs to differentiate into dendritic cells and macrophages by binding to phosphatidylserine on their cell surface. 2aG4.

Volume 17, Issue 2, Pages (February 2009)

Opposing Effects of TGF-β and IL-15 Cytokines Control the Number of Short-Lived Effector CD8+ T Cells Shomyseh Sanjabi, Munir M. Mosaheb, Richard A.

Volume 24, Issue 1, Pages (January 2006)

IL-6 neutralization results in induction of CD161+ FOXP3+ CD127– Tregs in EC–CD4+ T cell cocultures. IL-6 neutralization results in induction of CD161+

IL-6 neutralization decreases T cell proliferation and cytokine production upon restimulation by allogeneic HUVEC. CIITA transduced HUVECs were cocultured.

Donor and recipient BAL T cells are phenotypically and functionally memory T cells. Donor and recipient BAL T cells are phenotypically and functionally.

Volume 131, Issue 6, Pages (December 2006)

Anti-SEMA4D and anti–CTLA-4 treatment is synergistic and increases the frequency of tumor-specific TILs and secretion of proinflammatory cytokines, while.

PD-1 blockade enhances T-cell function.

CD25+ proliferation and suppression are independent functional variables in type 1 diabetes. CD25+ proliferation and suppression are independent functional.

T-CTLs are CD8+ TEMRA cells that can be expanded by IL-15 and IL-2 through selective proliferation and can be induced by LCs. T-CTLs are CD8+ TEMRA cells.

CD4+CLA+CD103+T cells from human blood and skin share a functional profile. CD4+CLA+CD103+T cells from human blood and skin share a functional profile.

Shared phenotype of CD4+CLA+CD103+ T cells from human blood and skin.

Dox titration and kinetic analyses of CD19CAR expression upon Dox administration and discontinuation. Dox titration and kinetic analyses of CD19CAR expression.

Volume 36, Issue 5, Pages (May 2012)

M-MDSC:mast cell cross-talk in the regulation of TNFα production.

Reduced tumor growth in CCR5-deficient mice is associated with perturbed killing ability of Treg cells. Reduced tumor growth in CCR5-deficient mice is.

CD11bhighCD27high conventional NK cells are converted into MDSCs

Fig. 2 Phenotypic analyses of Bcl11b-deficient Treg cells.

The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle distribution. The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle.

A, iDC (top) or mDC (bottom) were loaded with or without 10 pfu/cell reovirus, and cultured with Mel-888 cells in the presence of blocking serum. A, iDC.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Canonical but not adaptive NK-cell function is suppressed by Tregs.

CD19-CAR-T cells with short and long spacers show specific in vitro function. CD19-CAR-T cells with short and long spacers show specific in vitro function.

Treg expression of Gata3 plays a major role in controlling dermal fibrosis. Treg expression of Gata3 plays a major role in controlling dermal fibrosis.

Tregs preferentially regulate TH2 cytokines in skin.

DAC treatment alters immune cell composition and enhances cytokine production in the peritoneal lavage. DAC treatment alters immune cell composition and.

PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate distinct mechanisms of regulation. PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate.

Immune checkpoint molecule expression in primary and secondary tumors following radiotherapy. Immune checkpoint molecule expression in primary and secondary.

LSECtin, expressed by B16 cells, inhibits the tumor-specific immune responses both in vivo and in vitro. LSECtin, expressed by B16 cells, inhibits the.

LDL cholesterol inhibits Vγ9Vδ2 T-cell activation and cytokine production. LDL cholesterol inhibits Vγ9Vδ2 T-cell activation and cytokine production. Preexpanded.

IL35 regulation of tumor growth is accompanied by suppression of CD4+ effector T-cell activity and expansion of Tregs. IL35 regulation of tumor growth.

The effect of βAR signaling on the generation of a cytotoxic CD8+ T-cell response in vivo. The effect of βAR signaling on the generation of a cytotoxic.

Volume 20, Issue 6, Pages (June 2004)

Comparison of MLTC responses to tumor/peptide stimuli at day 18 and day 34 of growth. Comparison of MLTC responses to tumor/peptide stimuli at day 18 and.

EC-derived SP cells are targeted by CD30.CAR T cells.

Varying the MHC-I affinity, TCR affinity or antigen dose alters the phenotype of CD8 T cells ex vivo. Varying the MHC-I affinity, TCR affinity or antigen.

Presentation transcript:

cHL cell lines induce a suppressive Treg phenotype in cocultured CD4+ T cells. cHL cell lines induce a suppressive Treg phenotype in cocultured CD4+ T cells. A, Representative examples of induction of CD25hiCD127− Tregs after coculture with cHL (L428, SUP-HD1) or NHL (SUD-HL4) cell lines after 72 hours. P1 indicates a natural Treg population in single T-cell culture; P2 indicates bulk CD4+ T cells after depletion of CD25+ T cells, P3 marks the induced Treg population after cocultivation with L428 cells. B, Summary of coculture experiments. Shown is the mean ± SD. C, qRT-PCR of isolated nTreg (P1) subsets and induced Tregs (P3) relative to the CD25-depleted population P2 as indicated in (A). D, Flow cytometric analysis of Treg cell markers in CD4+ T cells isolated after coculture. E, Flow cytometric analysis of intracellular staining of typical Th1 cytokines after coculture and subsequent stimulation with PMA/ionomycin. Shown is the percentage of positive cells among CD4+ T cells. F, Representative example of a suppression assay using HRS cell-primed T cells and autologous, CD3+ CFSE-labeled RC. Displayed are the conditions of coculture of nonprimed T cells with RC under incomplete stimulation, coculture of nonprimed T cells and RC, coculture of HRS cell-primed T cells and RC, and coculture of a CD25+ subpopulation of HRS cell-primed T cells and RC. Except for the last condition, CD25+ T cells were depleted prior to coculture with HRS cells. CD8+ RC were gated to monitor proliferation during TCR engagement. G, Summary of suppression assay results. All experiments were performed independently at least three times. HRS cell–T-cell cocultures lasted typically for 3 days. *, P < 0.05. Shown is the mean ± SD. Frederik Wein et al. Cancer Immunol Res 2017;5:1122-1132 ©2017 by American Association for Cancer Research