Sodium nitroprusside treatment during the superovulation process improves ovarian response and ovarian expression of vascular endothelial growth factor.

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Presentation transcript:

Sodium nitroprusside treatment during the superovulation process improves ovarian response and ovarian expression of vascular endothelial growth factor in aged female mice Dong-Hyung Lee, M.D., Bo-Sun Joo, Ph.D., Dong-Soo Suh, M.D., Jong-Hun Park, M.D., Young-Min Choi, M.D., Kyu-Sup Lee, M.D. Fertility and Sterility Volume 89, Issue 5, Pages 1514-1521 (May 2008) DOI: 10.1016/j.fertnstert.2007.05.067 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Western blot analysis of vascular endothelial growth factor (VEGF) expression in the ovaries of different aged mice. The ovaries were isolated just after the flushing of one-cell embryos. (A) 16-week-old mice. (B) 26-week-old mice. Lane 1: PMSG alone (control). Lane 2: 1 μM SNP + PMSG. Lane 3: 10 μM SNP + PMSG. Fertility and Sterility 2008 89, 1514-1521DOI: (10.1016/j.fertnstert.2007.05.067) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Immunohistochemistry of vascular endothelial growth factor (VEGF) expression in ovaries of mice aged 26 weeks. The ovaries were isolated just after the flushing of one-cell embryos. Immunostaining with anti-VEGF antibody was usually localized in granulosa cells (Gr), stromal cells (St), and endothelial cells (Ec). The immunostaining intensity was increased after sodium nitroprusside (SNP) treatment. (A) PMSG alone (control). (B) 1 μM SNP + PMSG. (C) 10 μM SNP + PMSG (×100). Fertility and Sterility 2008 89, 1514-1521DOI: (10.1016/j.fertnstert.2007.05.067) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions