Staphylococcal exotoxins exert proinflammatory effects through inhibition of eosinophil apoptosis, increased surface antigen expression (CD11b, CD45,

Slides:

Advertisements

Similar presentations

Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions Kathleen R. Bartemes, BA, Kate.

Advertisements

Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila.

Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response Miriam Wittmann, MD, Mareike Alter, Tanja.

Impaired Responses of Peripheral Blood Mononuclear Cells to Staphylococcal Superantigen in Patients with Severe Atopic Dermatitis: A Role of T Cell Apoptosis

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Clemens Feistritzer, MD, Birgit A. Mosheimer, MD, Daniel H

Effect of interleukin-5 and granulocyte-macrophage colony stimulating factor on in vitro eosinophil function: Comparison with airway eosinophils Julie.

Role of IL-9 in the pathophysiology of allergic diseases

Estrogen enhances immunoglobulin production by human PBMCs

Glycation of a food allergen by the Maillard reaction enhances its T-cell immunogenicity: Role of macrophage scavenger receptor class A type I and II

Prostaglandin E2 control of T cell cytokine production is functionally related to the reduced lymphocyte proliferation in atopic dermatitis Sai Chan,

Histamine in the immune regulation of allergic inflammation

Human dendritic cell subset 4 (DC4) correlates to a subset of CD14dim/−CD16++ monocytes Federica Calzetti, BS, Nicola Tamassia, PhD, Alessandra Micheletti,

Toll-like receptor 9 suppression in plasmacytoid dendritic cells after IgE-dependent activation is mediated by autocrine TNF-α John T. Schroeder, PhD,

Β-Adrenergic regulation of the eosinophil respiratory burst as detected by lucigenin- dependent luminescence Nicholas E. Hadjokas, PhD a,b, Joseph J.

Requirements for allergen-induced airway inflammation and hyperreactivity in CD4- deficient and CD4-sufficient HLA-DQ transgenic mice Svetlana P. Chapoval,

Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A Satoshi Matsukura, MD, Fumio Kokubu,

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent Brittany M. Salter, BSc, John Paul Oliveria,

Mark Gorelik, MD, Satya D. Narisety, MD, Anthony L

Cristiana Stellato, MD, PhDa, Jun Atsuta, MD, PhDb, Carol A

David M. Pyle, BS, Victoria S. Yang, MD, Rebecca S

Glucocorticoids inhibit IL-4 and mitogen-induced IL-4Rα chain expression by different posttranscriptional mechanisms Lourdes Mozo, PhDa, Abel Gayo, BSa,

IL-31 Induces Chemotaxis, Calcium Mobilization, Release of Reactive Oxygen Species, and CCL26 in Eosinophils, Which Are Capable to Release IL-31 Nikola.

The effects of glucocorticoids on human eosinophils

The immunopharmacology of mild asthma

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Delayed eosinophil programmed cell death in vitro: A common feature of inhalant allergy and extrinsic and intrinsic atopic dermatitis Bettina Wedi, MD,

Chemokines induce eosinophil degranulation through CCR-3

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

Demonstration of circulating allergen-specific CD4+CD25highFoxp3+ T-regulatory cells in both nonatopic and atopic individuals Laura Maggi, BSc, Veronica.

Staphylococcal exotoxins are strong inducers of IL-22: A potential role in atopic dermatitis Margarete Niebuhr, MD, Helena Scharonow, MS, Merle Gathmann,

Human epidermal Langerhans' cells are targets for the immunosuppressive macrolide tacrolimus (FK506) Andrea Panhans-Groß, MD, Natalija Novak, MD, Stefan.

Protease-dependent activation of epithelial cells by fungal allergens leads to morphologic changes and cytokine production Henk F. Kauffman, PhD, J.F.Chris.

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Eosinophilia and gastrointestinal symptoms after ingestion of shiitake mushrooms Aaron M. Levy, MDa, Hirohito Kita, MDb, Sidney F. Phillips, MDa, Paul.

Ganglioside GQ1b enhances Ig production by human PBMCs

Serotonin Activates Human Monocytes and Prevents Apoptosis

Ganglioside GQ1b enhances anti–double-stranded DNA antibody and IgG production of PBMCs from patients with systemic lupus erythematosus Naoko Kanda,

Glucocorticoid-induced surface expression of annexin 1 blocks β2-integrin adhesion of human eosinophils to intercellular adhesion molecule 1 surrogate.

Antigen-specific and nonspecific determinants of cytokine production during topical sensitization of mice to chemical allergens Artin Moussavi, PhDa,

TH1/TH2 cytokines and inflammatory cells in skin biopsy specimens from patients with chronic idiopathic urticaria: Comparison with the allergen-induced.

Fetal and neonatal IL-13 production during pregnancy and at birth and subsequent development of atopic symptoms Toby J. Williams, Catherine A. Jones,

Human alveolar epithelial cells engulf apoptotic eosinophils by means of integrin- and phosphatidylserine receptor-dependent mechanisms: A process upregulated.

P38 Mitogen-activated protein kinase–induced glucocorticoid receptor phosphorylation reduces its activity: Role in steroid-insensitive asthma Elvis Irusen,

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells Iris Bellinghausen,

Acquisition and alteration of adhesion molecules during cultured human mast cell differentiation Hiroshi Tachimoto, MD, PhD, Sherry A. Hudson, MSB, Bruce.

Toll-like receptor 2 ligands activate human basophils for both IgE-dependent and IgE- independent secretion Anja P. Bieneman, BS, Kristin L. Chichester,

Peter J. Barnes, DM, DSc, FRCP

Larry L. Thomas, PhD, Michelle D. Haskell, Emmanuel U

Inhibition of protein kinases A and C demonstrates dual modes of response in human eosinophils stimulated with platelet-activating factor Takumi Takizawa,

Delayed eosinophil apoptosis in asthma

Qianli Zhuang, MD, PhD, Bruce Mazer, MD

Chemical constituents of diesel exhaust particles induce IL-4 production and histamine release by human basophils Gilles Devouassoux, MDa, Andrew Saxon,

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Thymic stromal lymphopoietin and OX40 ligand pathway in the initiation of dendritic cell–mediated allergic inflammation Yong-Jun Liu, MD, PhD Journal.

Pia J. Hauk, MDa, Qutayba A. Hamid, MD, PhDc, George P

Dose-dependent agonist and antagonist effects of the platelet-activating factor analogue 1-palmitoyl-2-acetoyl-sn-glycero-3-phosphocholine on B lymphocytes

Robert P. Schleimer, PhD Journal of Allergy and Clinical Immunology

Abnormalities of cell and mediator levels in bronchoalveolar lavage fluid of patients with mild asthma Sally E. Wenzel, MD Journal of Allergy and Clinical.

Lactic acid bacteria inhibit TH2 cytokine production by mononuclear cells from allergic patients Pierre Pochard, PhDab, Philippe Gosset, PhDb, Corinne.

Mechanisms of inhibition of IgE synthesis by nedocromil sodium: Nedocromil sodium inhibits deletional switch recombination in human B cells Richard K.S.

The novel synthetic immune response modifier R-848 (Resiquimod) shifts human allergen-specific CD4+ TH2 lymphocytes into IFN-γ–producing cells Francesca.

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.

Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring.

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Presentation transcript:

Staphylococcal exotoxins exert proinflammatory effects through inhibition of eosinophil apoptosis, increased surface antigen expression (CD11b, CD45, CD54, and CD69), and enhanced cytokine-activated oxidative burst, thereby triggering allergic inflammatory reactions Bettina Wedi, MD, Dorothea Wieczorek, Tanja Stünkel, Kristine Breuer, MD, Alexander Kapp, MD Journal of Allergy and Clinical Immunology Volume 109, Issue 3, Pages 477-484 (March 2002) DOI: 10.1067/mai.2002.121702 Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 1 Preincubation with polymyxin B-;blocked, LPS-mediated (but not SET-mediated) inhibition of eosinophil apoptosis. LPS in the concentrations indicated (in nanograms per milliliter) or SEA, SEB, SEC, or TSST-1 (1 μg/mL each) was preincubated for 10 minutes with (+ PB) or without (− PB) polymyxin B (50 U/mL) and thereafter incubated at 37°C in 5% CO2 for 48 hours. Data are presented as means ± SD of 4 experiments. *P < .05, **P < .01 versus respective value without polymyxin B. Journal of Allergy and Clinical Immunology 2002 109, 477-484DOI: (10.1067/mai.2002.121702) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 2 A , SET-induced inhibition of spontaneous eosinophil apoptosis in comparison with that induced by IL-3. Eosinophils were incubated with medium (Med) , IL-3 (10 ng/mL) or SEA, SEB, SEC, or TSST-1 in the concentrations indicated (in micrograms per milliliter) for 24 (filled columns) , 48 (open columns) , or 72 (shaded columns) hours. B , Inhibition of anti-Fas mAb-;induced eosinophil apoptosis by IL-3 and SETs. Eosinophils were incubated for 24 (filled columns) , 48 (open columns) , or 72 (shaded columns) hours with anti-Fas mAb (2 μg/mL) and the indicated stimulus: medium (Med) , IL-3 (10 ng/mL), SEA (1 μg/mL), SEB (5 μg/mL), SEC (5 μg/mL), or TSST-1 (5 μg/mL). Data are presented as means ± SEM of 11 experiments with eosinophils from different donors. *P < .05, **P < .01, ***P < .001 versus respective medium value. Journal of Allergy and Clinical Immunology 2002 109, 477-484DOI: (10.1067/mai.2002.121702) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 3 IL-3 and SEB upregulated the eosinophil activation marker CD69. Eosinophils were stimulated for 24 hours with control medium, IL-3 (10 mg/mL), SEB (1 μg/mL or 5 μg/mL), or LPS (10 ng/mL) before surface expression of CD69 (thick line) , and the respective isotype control (thin line) was assessed by means of flow cytometric analysis. The experiment is representative of 8 different experiments. In contrast, the other SETs (ie, SEA, SEC, and TSST-1) were ineffective. For P values, see Table II. Journal of Allergy and Clinical Immunology 2002 109, 477-484DOI: (10.1067/mai.2002.121702) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 4 Time course of GM-CSF-;enhanced eosinophil oxidative burst after 24 hours' prestimulation with SETs. Eosinophils were preincubated for 24 hours with SEA, SEB, SEC, or TSST-1 (5 μg/mL each) or LPS (10 ng/mL) and thereafter directly activated with GM-CSF (10 ng/mL). Integral counts were obtained from a 0- to 60-minute incubation interval immediately after addition of cytokines and were indicated as intensity counts ×10−3. The experiment is representative of 5 different experiments. For P values of all experiments, see Table III. Journal of Allergy and Clinical Immunology 2002 109, 477-484DOI: (10.1067/mai.2002.121702) Copyright © 2002 Mosby, Inc. Terms and Conditions