Volume 29, Issue 1, Pages (January 2008)

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Volume 29, Issue 1, Pages 122-133 (January 2008) Crystal Structure of Bacillus stearothermophilus UvrA Provides Insight into ATP- Modulated Dimerization, UvrB Interaction, and DNA Binding  Danaya Pakotiprapha, Yoshihiko Inuzuka, Brian R. Bowman, Geri F. Moolenaar, Nora Goosen, David Jeruzalmi, Gregory L. Verdine  Molecular Cell  Volume 29, Issue 1, Pages 122-133 (January 2008) DOI: 10.1016/j.molcel.2007.10.026 Copyright © 2008 Elsevier Inc. Terms and Conditions

Figure 1 Structure of Bacillus stearothermophilus UvrA (A) Overall structure of the UvrA monomer, with α helices depicted as cylinders and β strands as arrows. The protein is colored by domains, ATP-binding I (1–87, 503–590), red; signature I (88–117, 257–286, 399–502), pink; ATP-binding II (609–686, 843–952), blue; signature II (687–842), cyan; UvrB-binding (118–256), yellow; insertion (287–398), green; and linker (591–608), gray; with the Zn atoms, numbered by module, in light green. The bound ADP molecules are shown as space-filling models. The location of each domain, colored as above, is projected onto the primary sequence of BstUvrA, shown as a bar. (B) Overall structure of the UvrA dimer as observed in the crystal asymmetric unit. One protomer is colored as in (A) and the second protomer in gray. (C) Secondary structure assignment of BstUvrA, colored by domains as above. Disordered regions are depicted as dashed lines. Locations of the conserved ABC ATPase motifs, glycine-rich loops, and Zn-coordinating residues are depicted on the amino acid sequence. Molecular Cell 2008 29, 122-133DOI: (10.1016/j.molcel.2007.10.026) Copyright © 2008 Elsevier Inc. Terms and Conditions

Figure 2 Structural Comparison of the NBDs of BstUvrA and Other ABC ATPases The conserved ATPase motifs are colored as follows: Walker A/P loop, green; Walker B and D loop, orange; ABC signature motif, blue; Q loop, magenta; and H loop/switch, cyan. (A) Arrangement of the NBDs in E. coli MalK, Pyrococcus furiosus RLI, and BstUvrA. Key residues important for nucleotide binding and interactions across the dimer interface are shown. The transmembrane portion of MalK is depicted in transparent yellow. (B) NBDs of UvrA were superimposed with the NBDs of the maltose transporter MalK (ADP-bound, PDB code 2AWO; ATP-bound, PDB code 1Q12), and Rad50 (ATP-bound, PDB code 1F2U) using their respective ATP-binding domains. All the motifs are from the same NBD except for the ABC signature motif and the D loop, which are part of the opposing NBD. The bound ADP is from the proximal site of UvrA protomer A and is represented as ball and stick. (C) Histogram showing the distance between the Cα atoms of the conserved Lys residue in the Walker A motif and Ser residue in the ABC signature motif in the structures of ABC ATPases solved in the dimeric state (PDB codes 2R6F, 2AWO, 1Q1B, 1Q1E, 2AWN, 1YQT, 1L7V, 2ONK, 1Q12, 1F2U, 1XEF, 1XEX). Molecular Cell 2008 29, 122-133DOI: (10.1016/j.molcel.2007.10.026) Copyright © 2008 Elsevier Inc. Terms and Conditions

Figure 3 Nucleotide Binding by BstUvrA (A) Binding of ADP by the Walker A motif. ADP and the interacting protein residues are shown as sticks, with the unknown solvent component, modeled as a water molecule, depicted as a red sphere. Hydrogen bonds are drawn as dashed lines. The difference electron density calculated with the nucleotide and water molecule omitted from the model is shown at 3σ. (B) Comparison of the nucleotide-binding sites from UvrA (left) and MalK (right, PDB code 2AWO). The ATPase motifs are colored as in Figure 2. Important conserved residues are shown as sticks. The conserved glycine-rich loop found in UvrA is peach. All four nucleotide-binding sites in the UvrA dimer are structurally very similar; thus, only the proximal site of protomer A is shown. Molecular Cell 2008 29, 122-133DOI: (10.1016/j.molcel.2007.10.026) Copyright © 2008 Elsevier Inc. Terms and Conditions

Figure 4 BstUvrA Dimer Interface Cα trace of protomers A and B are shown in pale green and pale blue, respectively, with the Zn atoms in gray. The regions involved in polar contacts across the dimer interface are shown using ribbon diagram (green and orange, protomer A; blue and magenta, protomer B). Hydrogen bonds are depicted as dashed lines and the bound ADP molecules as space-filling models. Illustrations in (A) –(C) are shown in the same orientation as in Figure 1B. (A) UvrA dimer. (B) Interactions between the Q loop-I and the loop following α helix 1, which contains the Walker A-I motif at its N terminus. (C) Interactions between the loop preceding Walker B-I and residues of the ATP-binding domain I of the opposing monomer. Molecular Cell 2008 29, 122-133DOI: (10.1016/j.molcel.2007.10.026) Copyright © 2008 Elsevier Inc. Terms and Conditions

Figure 5 Biochemical Characterizations of BstUvrA Mutants The deleted residues are indicated and the mutated regions in DNA-binding mutants 1–4 defined in Figures 6C and 6D. (A) Incision of the 5′ end-labeled 50 bp duplex containing the N3-menthol lesion to yield a 19 bp product. (B) ATP hydrolysis as monitored by the coupled enzyme system (see the Supplemental Experimental Procedures), reported as mean turnover number (kcat) (mol ATP/min/mol UvrA) ± standard error of the mean (n = 8). (C) UvrA-UvrB interaction as assessed by size-exclusion chromatography. (D) Binding of UvrA and UvrB to the 50 bp duplex containing the N3-menthol lesion. The position of the UvrA·DNA, UvrAB·DNA, and UvrB·DNA complexes are indicated. Molecular Cell 2008 29, 122-133DOI: (10.1016/j.molcel.2007.10.026) Copyright © 2008 Elsevier Inc. Terms and Conditions

Figure 6 A DNA-Binding Model for UvrA as Inferred from Sequence Conservation and Electrostatic Surface Potential The ventral surface of the UvrA dimer is shown in the same view as in Figure 1B, while the dorsal surface is rotated 180° about a vertical axis. The structurally diverse region (SDR), which is part of the signature domain II, is circled. (A) Sequence conservation index was calculated and projected onto BstUvrA molecular surface. The color ranges from yellow (60% conservation) to dark green (≥96% conservation). Residues with <60% conservation are colored white. (B) Electrostatic potential for BstUvrA. The colors range from red (corresponding to an electrostatic potential energy of −10 kBT, where kB is the Boltzmann's constant and T is the temperature) to blue (+10 kBT). (C) Sequence conservation and surface electrostatics in combination with biochemical analysis permit the approximate path for DNA to be defined. Biochemical studies were performed on wild-type and mutant UvrA containing K→A/R→A substitutions at the positions depicted in colors; the rest of the protein is shown in gray. The path of the DNA is drawn in transparent yellow. (D) Summary of biochemical analyses of the mutants. Molecular Cell 2008 29, 122-133DOI: (10.1016/j.molcel.2007.10.026) Copyright © 2008 Elsevier Inc. Terms and Conditions