Sequence variation of 16S rRNA gene primer-binding sites.

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Sequence variation of 16S rRNA gene primer-binding sites. Sequence variation of 16S rRNA gene primer-binding sites. (A) Schematic of the 16S rRNA gene regions and primer pairs. (B) Occurrences of the most commonly observed sequences of primer-binding sites and the primer formulations that they may match. Nucleotide sites identical to the most common consensus sequence (the first listed) are represented as dots. The main nucleotide changes of primer sequences from the most common consensus sequence are highlighted in red. Zigui Chen et al. mSystems 2019; doi:10.1128/mSystems.00271-18