Diabetes perturbs the myeloid cell composition in wounded mice.

Slides:

Advertisements

Similar presentations

1 Supplementary figure 1 CD64MHCIIF4/80CD24CD11c FMO Macrophages CD11b + DCs CD103 + DCs Steady state b c Hh +anti-IL10R uninfected ** n.s.

Advertisements

S2 Fig. Phenotype of donor cells in the spleen and bone marrow of male mice. Splenocytes and bone marrow were isolated from male donor mice and stained.

Volume 16, Issue 6, Pages (August 2016)

Glycoprotein Nonmelanoma Clone B Regulates the Crosstalk between Macrophages and Mesenchymal Stem Cells toward Wound Repair Bing Yu, Talib Alboslemy,

Volume 6, Issue 5, Pages (November 2009)

Effects on the content of mtDNA and the expression of the OXPHOS genes by deletion of SHMT2 in 293A cells. Effects on the content of mtDNA and the expression.

Volume 41, Issue 1, Pages (July 2014)

by Dior Kingston, Michael A

Volume 21, Issue 13, Pages (December 2017)

Effects of AG-221 treatment on survival and cell differentiation in an IDH2R140Q primary human AML xenograft model. Effects of AG-221 treatment on survival.

Insulin content of isolated pancreas and islet morphology.

INT-767 treatment prevents renal lipid accumulation in diabetic DBA/2J mice. INT-767 treatment prevents renal lipid accumulation in diabetic DBA/2J mice.

Depo-Provera altered the expression of cell surface markers associated with HIV susceptibility. Depo-Provera altered the expression of cell surface markers.

Functional Beta2-Integrins Restrict Skin Inflammation In Vivo

Increased chemokine content and leukocyte infiltrate in D6-negative tumors. Increased chemokine content and leukocyte infiltrate in D6-negative tumors.

CCL21 localizes to T cell zone FRCs in young and aged spleens.

Volume 7, Issue 4, Pages (October 2016)

Volume 142, Issue 2, Pages (February 2012)

CD169+ macrophages play a critical role in mediating innate immune cell reorganization. CD169+ macrophages play a critical role in mediating innate immune.

Volume 39, Issue 5, Pages (November 2013)

Fig. 5 Hypoxic tumors from obese mice associate with increased production of IL-6 by adipocytes and myeloid cells. Hypoxic tumors from obese mice associate.

Volume 12, Issue 11, Pages (September 2015)

GM-CSF–producing γδT cells are involved in the development of EAM

IL-21 is involved in the pathogenesis of EAM

CCR2+ monocytes are a relevant source of type I IFN in response to Af.

Conditional deletion of MHC II on B cells does not regulate atherosclerotic plaque development in Ldlr−/− mice. Conditional deletion of MHC II on B cells.

Volume 6, Issue 5, Pages (November 2009)

Conditional expression of MHC II on B cells does not regulation atherosclerotic plaque development in Ldlr−/− mice. Conditional expression of MHC II on.

Volume 47, Issue 5, Pages e4 (November 2017)

Immune cell populations, activation, and NKG2D and H60a expression in the spleen of untreated and antibiotic-treated Klrk1+/+ and Klrk1−/− NOD mice. Immune.

Volume 85, Issue 4, Pages (February 2015)

2aG4 directly induces monocytic MDSCs to differentiate into dendritic cells and macrophages by binding to phosphatidylserine on their cell surface. 2aG4.

Melissa B. Uccellini, Adolfo García-Sastre Cell Reports

VEGF-A concentration is increased in vivo in response to DC101 and in vitro in response to FG VEGF-A concentration is increased in vivo in response.

Discordant expression of Foxp3 in induced Tregs.

Volume 34, Issue 5, Pages (May 2011)

B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo. B cells infiltrate mouse and human pancreatic neoplasia.

IL-27 contributes to IR expression by lung T cells during toxoplasmosis. IL-27 contributes to IR expression by lung T cells during toxoplasmosis. (A) Il27p28-GFP.

Neutrophil recruitment to the colonic lamina propria depends on CD4+ T cells. Neutrophil recruitment to the colonic lamina propria depends on CD4+ T cells.

Total and differential cell counting in the broncho-alveolar lavage of mice exposed to CS or air during 3 or 6 months. Total and differential cell counting.

Volume 38, Issue 2, Pages (February 2013)

PD-L1 selectively marks circulating NCMs.

Fig. 5 Hypoxic tumors from obese mice associate with increased production of IL-6 by adipocytes and myeloid cells. Hypoxic tumors from obese mice associate.

NRP1-expressing macrophages accumulate in adipose tissue during DIO and regulate weight gain and glucose tolerance. NRP1-expressing macrophages accumulate.

α-Cells mainly express SSTR2 and SSTR3

Fig. 4. The effect of combined inhibition of BCL-2 and BCR-ABL on leukemia LT-HSC frequency. The effect of combined inhibition of BCL-2 and BCR-ABL on.

VE-cadherin protein and mRNA are decreased in the retinal vessels of diabetic rats. VE-cadherin protein and mRNA are decreased in the retinal vessels of.

COX-2 deletion in macrophages led to increased renal macrophage infiltration in diabetic mice. COX-2 deletion in macrophages led to increased renal macrophage.

Diclofenac treatment reduces L-selectin on peripheral blood and infiltrated leukocytes but does not alter accumulation in the injured spinal cord. Diclofenac.

Major hematopoietic cell populations in NOD mice are not affected by c-Rel deficiency. Major hematopoietic cell populations in NOD mice are not affected.

PEDF inhibits Wnt/T-cell factor/β-catenin signaling in resting and wounded skin. PEDF inhibits Wnt/T-cell factor/β-catenin signaling in resting and wounded.

RORα deficiency in Tregs results in exaggerated skin inflammation in response to EC sensitization. RORα deficiency in Tregs results in exaggerated skin.

Induction of MHC-mismatched mixed chimerism depletes mature but not immature BM B cells by 60 days after HCT. Control and mixed chimeric NOD mice were.

ADCY5 is expressed in isolated human islets and affected by T2D risk alleles. ADCY5 is expressed in isolated human islets and affected by T2D risk alleles.

Cx3CR1-GFP expression identifies myeloid subpopulations in the cutaneous wound. Cx3CR1-GFP expression identifies myeloid subpopulations in the cutaneous.

Double-immunofluorescence labeling of Arg-1 in WT (A) and IL-10−/− (C) mice at 3 (i) and 7 (ii) days postinjury (dpi). Double-immunofluorescence labeling.

BM transplants identify Cx3CR1-GFP hi cells as resident macrophages.

by Ganesh V. Halade, Paul C. Norris, Vasundhara Kain, Charles N

Treatment with R848 and anti-CD200R leads to changes in the composition of intratumor myeloid cells. Treatment with R848 and anti-CD200R leads to changes.

Inflammation Mediated by JNK in Myeloid Cells Promotes the Development of Hepatitis and Hepatocellular Carcinoma Myoung Sook Han, Tamera Barrett, Michael.

CD25 expression predicts effector and memory differentiation.

Volume 10, Issue 7, Pages (February 2015)

Representative macrophage staining images only from the knee joints of CAIA mice injected s.c. with GalNAc–MASP-3–siRNA duplex 2 or GalNAc–luciferase–siRNA.

Indirect impression smear of material expressed from a furuncle stained with a modified Wright-Giemsa stain (1000× immersion oil). Indirect impression.

Fig. 4 Adenosine A2BR signaling promote osteogenic and inhibit osteoclast differentiation in vitro. Adenosine A2BR signaling promote osteogenic and inhibit.

Treg expression of Gata3 plays a major role in controlling dermal fibrosis. Treg expression of Gata3 plays a major role in controlling dermal fibrosis.

Fig. 6 Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9 in vivo. Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9.

Mechanisms of FRC-dependent myeloid cell recruitment.

Expansion of omental FRCs and FALC remodeling after intraperitoneal exposure to bacterial antigen. Expansion of omental FRCs and FALC remodeling after.

Volume 27, Issue 5, Pages (May 2019)

Presentation transcript:

Diabetes perturbs the myeloid cell composition in wounded mice. Diabetes perturbs the myeloid cell composition in wounded mice. CD11b+Ly6G−CCR2+ BM monocytes isolated from diabetic (Db) and non-diabetic (NDb) mouse BM flushes of animals at days 4 and 7 postwounding. (A) BM monocytes from day 7 wounded nondiabetic and diabetic mice were stained for Geimsa. Scale bar, 10 μm. (B) MFI of CCR2 and GFP on BM monocytes from nondiabetic and diabetic mice at days 4 and 7 postwounding (*p < 0.05, **p < 0.01, n = 3). Ly6C+CCR2+ cells isolated from diabetic and nondiabetic cutaneous wounds as before. (C) Representative Giemsa stains for the GFP subsets: neg/lo [(Ci) neutrophils, (Cii) monocytes, (Ciii) macrophages]; int [(Civ) monocytes, (Cv) macrophages]; and hi populations [(Cvi) monocytes, (Cvii) macrophages] from day 7 wounded diabetic mice. Scale bar, 10 μm. (D) Cell counts for the GFP neg/lo, GFP int, and GFP hi populations (*p < 0.05, n = 3). (E) Relative expression of Adgre1 and Csf1r to Hist2h2 mRNA levels, and MFI for CD11b (*p < 0.05, n = 3). Matthew Burgess et al. ImmunoHorizons 2019;3:262-273 Copyright © 2019 The Authors