Quantitation of PCR products.

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Presentation transcript:

Quantitation of PCR products. Quantitation of PCR products. A: Logarithmic plot of fluorescence versus cycle number of perforin of rhesus monkey. B: Standard curve constructed with the perforin standards from 106 to 102 copies. The standard curve was constructed by plotting the logarithmic concentration of the standard versus the crossing points (cycle number). The error of the standard curve (ε) = 3.4353E-3 (ε = sum of squares of deviation/number of measuring points). Dongmei Han et al. Diabetes 2002;51:562-566 ©2002 by American Diabetes Association