Segments R1, R3, R6 and R7, and GTPase activity of Mfn2ΔTM are necessary for mitochondrial fragmentation in COS7 cells. Segments R1, R3, R6 and R7, and.

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Segments R1, R3, R6 and R7, and GTPase activity of Mfn2ΔTM are necessary for mitochondrial fragmentation in COS7 cells. Segments R1, R3, R6 and R7, and GTPase activity of Mfn2ΔTM are necessary for mitochondrial fragmentation in COS7 cells. (A) Schematic diagram of the Mfn2 deletion and point mutants used to analyze mitochondrial fragmentation, and percentage of transfected cells with fragmented mitochondria. All constructs are epitope-tagged with the 3 ×FLAG sequence present at the N terminus. Boxed C, predicted coiled-coil domain; gray, GTPase domain; black, predicted transmembrane domains. The G2 Mfn2-T130A mutation is equivalent to a mutation in the Ras GTPase that abolishes effector interactions. (B) COS7 cells were transiently transfected with expression vector for FLAG-Mfn2ΔTM (a,b) and FLAG-Mfn2ΔR4/R5/TM (c,d). After 16 hours, the cells were labeled with MitoTracker to visualize mitochondria (b,d), fixed, and stained with anti-FLAG antibody followed by Alexa Fluor 488-conjugated secondary antibody (a,c). The star indicates untransfected cell with normal reticular mitochondria. Bar, 20 μm. Shinji Honda et al. J Cell Sci 2005;118:3153-3161 © The Company of Biologists Limited 2005