Microarray immunoassay: Association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes Wayne G. Shreffler, MD,

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Presentation transcript:

Microarray immunoassay: Association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes Wayne G. Shreffler, MD, PhD, Kirsten Beyer, MD, Te-Hua Tearina Chu, PhD, A.Wesley Burks, MD, Hugh A. Sampson, MD Journal of Allergy and Clinical Immunology Volume 113, Issue 4, Pages 776-782 (April 2004) DOI: 10.1016/j.jaci.2003.12.588

Fig 1 The microarray immunoassay detects allergen-specific IgE. Peanut-sensitized patients (n=77, shaded boxes) versus control subjects (n=15, open boxes) have significantly higher levels of IgE, as detected by using this assay to rAra h 1, rAra h 2, and rAra h 3 (∗∗P<.001). Journal of Allergy and Clinical Immunology 2004 113, 776-782DOI: (10.1016/j.jaci.2003.12.588)

Fig 2 IgE epitope profiles. A, The frequency of recognition plotted against each peptide on the microarray ordered from N to C terminus of Ara h 1 (red), Ara h 2 (green), and Ara h 3 (blue; n=77). B, Eight individual patient IgE profiles. Journal of Allergy and Clinical Immunology 2004 113, 776-782DOI: (10.1016/j.jaci.2003.12.588)

Fig 3 Individuals with a history of more severe peanut-induced reactions recognize more peanut allergen epitopes. A, Individuals with a history of cutaneous symptoms (n=14) recognize fewer epitopes (median, 2; range, 0-20) compared with those with a history of reactions involving multiple organ systems (n=24; median, 9.5; range, 1-29; P<.01). B, The same patients have similar recognition of recombinant peanut allergens. Journal of Allergy and Clinical Immunology 2004 113, 776-782DOI: (10.1016/j.jaci.2003.12.588)

Fig 4 Patient sera recognizing a greater number of IgE epitopes induce more degranulation of passively sensitized cells. Pooled sera from patients with high levels of peanut-specific IgE (>100 kU/L; n=4 for each group) were generated from sera recognizing few epitopes (squares) versus sera recognizing many epitopes (triangles) and sera from control subjects not allergic to peanut (diamonds). Serum pools were used to passively sensitize human basophils from a nonatopic donor (A), an atopic donor not allergic to peanut (B), and the rat basophilic leukemia cell line expressing the human FcεRI (C). PE, Peanut. Journal of Allergy and Clinical Immunology 2004 113, 776-782DOI: (10.1016/j.jaci.2003.12.588)