Nutrient availability regulates growth of the X. laevis optic tectum.

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Nutrient availability regulates growth of the X. laevis optic tectum. Nutrient availability regulates growth of the X. laevis optic tectum. Experimental timeline is identical to that in Fig. 1. (A) Representative images of the midbrain from tadpoles from three selected time points. Dotted lines in top left panel are shown as an example to indicate area measured. Scale bar, 200 µm. (B) Plot of the largest cross-sectional area of the optic tectum in NR (blue squares), fed (green triangles) and DF (orange circles) groups, shown as means±s.e.m. and normalized to day 0. NR led to smaller tectum cross-sectional areas by day 3 relative to fed groups. DF on day 3 promoted growth, allowing midbrain size to catch up to that of fed animals. Cross-sectional area in NR animals (blue squares) reversed, with tectum sizes declining to below the day 0 baseline by day 7. Refer to Table 1 for data and Table 3 for corresponding statistics; n=500 animals total from two independent clutches, with 11–22 animals measured per group per time point. C. R. McKeown et al. J Exp Biol 2017;220:358-368 © 2017. Published by The Company of Biologists Ltd