MRP2 inhibition mitigates pulmonary burden and bacteremia following lung challenge with S. pneumoniae. MRP2 inhibition mitigates pulmonary burden and bacteremia.

Slides:



Advertisements
Similar presentations
Primary human MNCs and MDMs are targets of ZIKV infection.
Advertisements

Extranodal dissemination of non-Hodgkin lymphoma requires CD47 and is inhibited by anti-CD47 antibody therapy by Mark P. Chao, Chad Tang, Russell K. Pachynski,
AKR1C3 is a biomarker of sensitivity to PR-104 in preclinical models of T-cell acute lymphoblastic leukemia by Donya Moradi Manesh, Jad El-Hoss, Kathryn.
IL-22 exacerbates weight loss in a murine model of chronic pulmonary Pseudomonas aeruginosa infection  Hannah K. Bayes, Neil D. Ritchie, Christopher Ward,
by Éric Aubin, Réal Lemieux, and Renée Bazin
Jennifer R. Hamilton, Gayathri Vijayakumar, Peter Palese  Cell Reports 
Combined PLX3397 and PTX treatment inhibits metastasis in a CD8-dependent manner. Combined PLX3397 and PTX treatment inhibits metastasis in a CD8-dependent.
In vivo responses of AMLMLL to ATRi.
Bay induced aggregation of Cp occurs in HBV-infected HepG2-NTCP cells.
Volume 15, Issue 2, Pages (February 2014)
Treatment of SFTSV-infected IFNAR−/− mice with T-705 or ribavirin.
Role of monocytes in NK cell activity.
Anti-Pseudomonas aeruginosa IgY antibodies augment bacterial clearance in a murine pneumonia model  K. Thomsen, L. Christophersen, T. Bjarnsholt, P.Ø.
Fig. 4. In vivo analysis of slpA mutant in the Syrian Golden hamster.
Volume 13, Issue 1, Pages (January 2006)
Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions
Fig. 5 Combination intravenous reovirus and checkpoint inhibition in an orthotopic syngeneic brain tumor model. Combination intravenous reovirus and checkpoint.
Neutrophil antifungal response in CCR2-depleted mice is rescued by adoptive transfer of CCR2+ monocytes or by treatment with recombinant IFNs. Neutrophil.
Mycobacterium tuberculosis Inhibits Neutrophil Apoptosis, Leading to Delayed Activation of Naive CD4 T cells  Robert Blomgran, Ludovic Desvignes, Volker.
The SOP can detect WNV infection in vitro and in vivo.
Volume 14, Issue 4, Pages (October 2013)
Fig. 7 BRD0705 impairs colony formation in AML cell lines and patient cells and shows in vivo efficacy in multiple AML mouse models. BRD0705 impairs colony.
FGF-2 signaling mediates expansion of HSPCs
Cytotoxic therapy induces CSF1-dependent macrophage recruitment.
Volume 26, Issue 1, Pages (January 2018)
Indomethacin worsens the effects of C. difficile infection in mice.
Fig. 5 Local gel scaffold for T cell memory response.
Volume 26, Issue 1, Pages (January 2018)
FepB is responsible for the smr mutant’s phenotype.
The pho4Δ mutant is less virulent than the WT strain in intranasal (A to C) and intravenous (D to F) models of cryptococcosis. The pho4Δ mutant is less.
Laura K. Certain, Jeffrey C. Way, Matthew J. Pezone, James J. Collins 
FLC treatment results in an increase in ploidy in a significant fraction of cells. FLC treatment results in an increase in ploidy in a significant fraction.
Volume 6, Issue 6, Pages (December 2009)
Platelet HMGB1 is required for efficient bacterial clearance in intra-abdominal bacterial sepsis in mice by Hui Zhou, Meihong Deng, Yingjie Liu, Chenxuan.
2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia by Elizabeth Tkaczynski, Abinaya Arulselvan, John Tkaczynski,
High-density foci of Giardia colonization are present in the proximal small intestine of mice and gerbils. High-density foci of Giardia colonization are.
Volume 15, Issue 3, Pages (March 2009)
CD40, but Not CD40L, Is Required for the Optimal Priming of T Cells and Control of Aerosol M. tuberculosis Infection  Vanja Lazarevic, Amy J Myers, Charles.
Volume 21, Issue 2, Pages (February 2017)
Reduction of neutrophil airspace influx does not significantly attenuate alveolar barrier disruption in thrombocytopenic Mpl−/− mice following either live.
Arp2/3-mediated formation of nuclear actin networks is essential for CD4+ T cell effector functions. Arp2/3-mediated formation of nuclear actin networks.
NK cell function of spleen MNCs (A) and NK cell frequencies in the ileum, duodenum, and spleen (B) of protein-deficient and -sufficient HIFM/HRV-infected.
Molecular Therapy - Oncolytics
A ID8 scr sh cx2 cx3 cx6 B C Supplementary Figure 4. The fractalkine axis does not regulate ascites formation.
Treatment of G. mellonella-infected larvae with norfloxacin or norfloxacin plus TPGS in combination with the wild type or ΔbcnA mutant. Treatment of G.
Supplementation of c-Rel–competent Treg cells reverts exacerbated diabetes in c-Rel−/− NOD mice. Supplementation of c-Rel–competent Treg cells reverts.
Prime and boosts with PBK001 vaccine provided 100% protection with low bacterial burden in organs. Prime and boosts with PBK001 vaccine provided 100% protection.
sIgM levels or signaling and FISH lesions in CLL
d-Serine utilization contributes to fitness of P
Stress resistance comparisons.
Intranasal immunization of mice with S
Dox administration was required for Tet-CD19CAR T cells to show a suppressive function against a CD19+ tumor in vivo. Dox administration was required for.
Primary B16F10 tumor inhibits experimental metastasis formation in the lung. Primary B16F10 tumor inhibits experimental metastasis formation in the lung.
Fig. 2 Adult LPL−/− mice that received neonatal rGM-CSF therapy are protected from pneumococcal infection. Adult LPL−/− mice that received neonatal rGM-CSF.
LEfSe comparison analysis between the control and ciprofloxacin or vancomycin-imipenem groups at the end of antibiotic treatment (A or B, respectively)
IDO deficiency delays the development of pulmonary metastases.
Neutrophil antifungal response in CCR2-depleted mice is rescued by adoptive transfer of CCR2+ monocytes or by treatment with recombinant IFNs. Neutrophil.
B. fragilis utilizes TLR2 signaling for its protective role against the development of colitis-associated cancer in mice. B. fragilis utilizes TLR2 signaling.
LAIV enhancement of pneumococcal density is time dependent and long lasting. LAIV enhancement of pneumococcal density is time dependent and long lasting.
Effect of MZ treatment on lung colony formation in an experimental metastasis. Effect of MZ treatment on lung colony formation in an experimental metastasis.
d-Serine utilization contributes to P
Pseudomonas PIV enhances pneumococcal pneumonia and bacteremia in C57BL/6 mice. Pseudomonas PIV enhances pneumococcal pneumonia and bacteremia in C57BL/6.
P. aeruginosa PIV and S. pneumoniae producing pneumolysin induce massive neutrophil recruitment and lung damage during active murine pneumonia. P. aeruginosa.
Colonization with B. fragilis protects mice against development of colon cancer. Colonization with B. fragilis protects mice against development of colon.
In vivo efficacy of JAK inhibition in transformed Pax5+/− pro-B cells harboring Jak3V670A. In vivo efficacy of JAK inhibition in transformed Pax5+/− pro-B.
ARQ 531 improves survival in the Eμ-TCL1 engraftment model compared with ibrutinib. ARQ 531 improves survival in the Eμ-TCL1 engraftment model compared.
GBM cells infected in vitro with HCMV divide less often than uninfected cells do. GBM cells infected in vitro with HCMV divide less often than uninfected.
Effect on the recall response of Nrp1 deletion before infection.
Parthenolide inhibits tumor promotion and increases p21 expression in vivo. Parthenolide inhibits tumor promotion and increases p21 expression in vivo.
Fig. 2. In vivo local CD25-targeted NIR-PIT induces regression of treated LL/2-luc tumors. In vivo local CD25-targeted NIR-PIT induces regression of treated.
Presentation transcript:

MRP2 inhibition mitigates pulmonary burden and bacteremia following lung challenge with S. pneumoniae. MRP2 inhibition mitigates pulmonary burden and bacteremia following lung challenge with S. pneumoniae. Using an in vivo model of PMN migration, we examined the results of MRP2 inhibition. C57BL/6 mice were treated with either PBS or probenecid 3 h before and 3 h after intratracheal application of 2.5 × 105 S. pneumoniae. Four sets of mice were infected in this way. (A) Comparing the number of PMNs found in the lumen after isolating BAL fluid samples after 24 and 48 h postinfection. PMNs, identified as Ly6g-positive cells, were quantified by flow cytometry (n = 8). (B) Overall lung burden from mice sacrificed on day 2. Mice were sacrificed, lungs were excised and homogenized, and total bacterial burden was calculated using serial dilutions (n = 24 for each condition). The presence (+) or absence (−) of bacteremia is indicated as follows: +, mice had detected levels of bacteria in blood 48 h postinfection; −, no bacteria were detected in blood during tail vein bleeds. (C) Bacteremia, as measured by tail vein bleeds, from the cohorts in panel B (see Materials and Methods). Detected events of colony formation on day 1 (D1) and day 2 (D2) (n = 24) are shown. The broken line represents the limit of detection, and as such, values for mice without visible bacteremia were represented as 100 CFU/ml, just below this level of detection. In panels B and C, statistical significance was calculated using the Mann-Whitney test. (D) Survival experiment with the fourth set of mice. There were 16 mice in a group for each condition. Statistical significance was calculated using Mantel-Cox test and Gehan-Breslow-Wilcoxon test. Probenecid treatment consistently increased survival by approximately 30 to 40% during survival experiments and often delayed symptoms, such as lethargy. Mouse experiments were repeated, and similar results were observed in at least two different experiments. Andrew Zukauskas et al. mSphere 2018; doi:10.1128/mSphere.00303-18