Biphasic increase of PI(4,5)P2 level in LPS-stimulated cells.

Slides:

Advertisements

Similar presentations

Figure 1. Effects of red ginseng extract on B cell proliferation and antibody production. Purified mouse splenic B cells were stimulated with LPS (1 µg/ml)

Advertisements

by Rosemary E. Smith, Vanshree Patel, Sandra D. Seatter, Maureen R

Specificity of production of reactive oxygen species (ROS) against Aspergillus fumigatus conidia by phagocytes from healthy and CF donors. Specificity.

TGF-β1 induces ILK activity in renal tubular epithelial cells.

The heat shock protein Gp96 binds to human neutrophils and monocytes and stimulates effector functions by Markus P. Radsak, Norbert Hilf, Harpreet Singh-Jasuja,

Alpha-synuclein induces autophagy in microglial cells.

Evaluation of LC3 and ATG13 dynamics in AS-stimulated microglial cells using live-imaging. Evaluation of LC3 and ATG13 dynamics in AS-stimulated microglial.

Fig. 3. Effect of NH4Cl (0 or 30 mM) on percentage of motile spermatozoa and VAP after 1 and 5 min after activation. Effect of NH4Cl (0 or 30 mM) on percentage.

Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. (A,B)

Effect of YopM on the transcription of pro-inflammatory cytokines in HL60-derived macrophages after stimulation with LPS. HL60 cells differentiated into.

Fig. 2. Immunopurified MRE11 removes topoisomerase IIα covalent complexes from genomic DNA.(A) Western blot probing K562 whole cell extract, K562 MRE11.

TWEAK downregulates SIRT3 in cultured tubular cells and this is prevented by crotonate. TWEAK downregulates SIRT3 in cultured tubular cells and this is.

The initial targeting of Sec61b mRNA to the ER is partially dependent on ribosomes and translation. The initial targeting of Sec61b mRNA to the ER is partially.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 5. Receptor tyrosine kinase activation in response to growth factor stimulation. Receptor tyrosine kinase activation in response to growth factor.

FAK tyrosine phosphorylation is unaffected by loss of LAR activity.

Fig. 1. Aboveground biomass of Caragana and herbaceous plants, and proportional abundance of Caragana, under different grazing management treatments. Aboveground.

G418 causes UPF1 to localize to cytoplasmic foci containing NMD substrates but not the P-body marker DCP1a. 6CFSMEo- cells were transfected with constructs.

In 6CFSMEo- cells, the NMD factor UPF1 colocalizes with NMD substrates under cytoskeleton disruptor treatment. 6CFSMEo- cells were transfected with constructs.

Fig. 1. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3.

GFP–Sec61b mRNA competes with t-ftz mRNA for the ribosome-binding sites on the ER. (A,B) COS7 cells were transfected with plasmid containing a test gene.

HSP90β interacts with HNF4A to regulate protein half-life.

α-toxin and PVL-mediated cytotoxicity is blocked by IVIG

Loss of LAR phosphatase activity is associated with decreased adhesion complex formation. Loss of LAR phosphatase activity is associated with decreased.

TWEAK increases histone crotonylation in kidney tubular cells.

Fig. 1. Expression of the five miRNAs encoded by two miRNA clusters in mouse sperm and oocytes.(A) qPCR analyses of levels of miR-16 (positive control),

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Reconstitution of GBF1 recruitment to Golgi membranes in a cell-free assay. Reconstitution of GBF1 recruitment to Golgi membranes in a cell-free assay.

Fig. 1. Phenotypic characterisation of primary human tubular epithelial cells and human renal fibroblasts. Phenotypic characterisation of primary human.

Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. Transbilayer distribution of cholesterol in.

Macrophage-produced IL-10 is required but not sufficient for macrophage-stimulated proliferation of ADPKD cells. Macrophage-produced IL-10 is required.

Expression analysis of periostin in DRG

The ABCG2-K86M mutant resides in the ER

Effect of cGMP on in vitro fluid secretion rate, cation (Na+ and K+) concentration and transport rate by adult female A. aegypti MTs stimulated with a.

The K86M mutation in ABCG2 results in a non-functional transporter.

The SIM domain of RAD51 is required for HR

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

Subdiffraction-limit imaging of BRCA1 and 53BP1 in IRIF

AT1R–EGFR transactivation occurs in HMEC-LST-AT1R cells.

Defective MEK-Erk1/2 kinase activation in RIAM-depleted cells accounts for their impaired FA disassembly. Defective MEK-Erk1/2 kinase activation in RIAM-depleted.

Graf-dependent GEEC endocytosis is required for EGFR internalization and degradation at high Spi. Graf-dependent GEEC endocytosis is required for EGFR.

Regulation of lipid droplet formation by PI3-kinase activity.

Stimulation of cells with FFAR4 agonist.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

Fig. 2. Effects of pH on percentage of motile spermatozoa and VAP after 1 and 5 min after activation. Effects of pH on percentage of motile spermatozoa.

miR-145 regulates IGF1R expression.

E4-ORF1 binds endogenous PATJ and redistributes it into detergent-insoluble complexes in MDCK cells. E4-ORF1 binds endogenous PATJ and redistributes it.

NmMlck is required for β-catenin and FoxO1 occupancy of the Cldn5 repressor. nmMlck is required for β-catenin and FoxO1 occupancy of the Cldn5 repressor.

The influence of SHIP2 on cell migration in breast cancer cells.

Clustering of CD14 in the plane of the plasma membrane of LPS-stimulated cells. Clustering of CD14 in the plane of the plasma membrane of LPS-stimulated.

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

Effect of PDGF-BB on mesenchymal mesonephric cell migration.

Amplitudes of Ca2+ puffs evoked by different IP3R subtypes.

pHSF1Ser230 is upregulated by TNF in colon cancer cells.

Ub-binding ability of ERα.

Expression of IP3R subtypes in HEK cells.

Characterization of the ERα-UBS.

Effect of siRNA transfection on colony formation by Renca cells.

p65 depletion increases basal protein aggregation and insolubilization

Effect of AedaeCAPA-1 on in vitro fluid secretion rate, cation (Na+ and K+) concentration and transport rate by adult female A. aegypti MTs stimulated.

Role of Src and PTP-PEST in upregulation of tyrosine phosphorylation of paxillin and FAK. (A) Mock and D11 cells were pre-incubated with PP2 or PP3 (10 µM,

Extracellular BMP modulators have different effects on endothelial cell sprouting in the tube formation assay. Extracellular BMP modulators have different.

Tsg protein stimulates endothelial cell migration, proliferation and collagen gel spheroid sprouting. Tsg protein stimulates endothelial cell migration,

Distinct mitochondrial membrane potential, ATP-to-ADP ratio and NAD(P)H responses to glucose and methylsuccinate. Distinct mitochondrial membrane potential,

GOLPH3 interacts with COG complex proteins.

Phenotype of S. pombe cells in the presence of B21P2 or LMB

Upon challenge with oxazolone, human leukocytes (mainly consisting of T cells) infiltrate the dermis and epidermis. Upon challenge with oxazolone, human.

Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

LPS-induced TNF-α secretion from differentiated and undifferentiated THP-1 cells. LPS-induced TNF-α secretion from differentiated and undifferentiated.

p110δ activity is important for autoantibody responses.

Presentation transcript:

Biphasic increase of PI(4,5)P2 level in LPS-stimulated cells. Biphasic increase of PI(4,5)P2 level in LPS-stimulated cells. J774 cells were stimulated for the indicated times with 10 ng/ml (A) or 100 ng/ml (B,C) of LPS with or without pre-incubation with 10 μg/ml of a function-blocking antibody against CD14. After extraction of phosphoinositides, PI(4,5)P2 amounts in samples were estimated using ELISA (A,B) or a protein–lipid overlay assay using the PLC-PH–GST probe, followed by densitometry analysis (presented in the histogram, C). (C, right) The results of the protein–lipid overlay assay, which was performed on lipids that had been extracted from unstimulated cells or cells stimulated with 100 ng/ml LPS for 5 and 60 min. Samples were incubated with the PLC-PH–GST probe, which had been pre-adsorbed with liposomes containing or devoid of PI(4,5)P2. Data are the mean±s.e.m. of three to five experiments. *P≤0.05, significantly different from unstimulated cells. Agnieszka Płóciennikowska et al. J Cell Sci 2015;128:4096-4111 © 2015. Published by The Company of Biologists Ltd