Fig. 6. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. Bj mutants show stereocilia patterning defects and biliary.

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Fig. 6. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. (A-D) Immunostaining with phalloidin (green) and Vangl2 (red) of the cochlea of near-term wild-type (A,C) and Bj mutant (B,D) embryo. The phalloidin staining delineated the normal chevron-shaped stereocilia in the outer hair cells (OHCs), showing malalignment in the Bj mutant (boxed B) compared to wild-type (boxed A). In addition, Vangl2 staining revealed a subtle misalignment of supporting cells connecting the inner hair cells (IHCs) in the Bj mutant (B), better seen in the enlarged views shown in panels C and D (see arrowheads). (E-J) Shown are bile ducts from a wild-type newborn mouse (E) (n=6) as compared to that of a Bj mutant (F) (n=6), which is noticeably shorter. Quantitative analysis showed a significant decrease in both the length and width of the Bj mutant biliary duct (G). Examination of hematoxylin and eosin stained sections of newborn wild-type (H) (n=4) and Bj mutant (I) biliary ducts suggest a decrease in the mucosal folds of the mutant duct (I) (n=5). This was confirmed with quantitative analysis, which showed a significant decrease in mucosal folds in the mutant duct (J). Error bars show standard deviation. (K-P) Immunostaining with antibodies against β-catenin (red) and E-cadherin (green) showed β-catenin expression is markedly reduced in the Bj mutant biliary duct (N,P), but no change was observed for E-Cadherin (O,P,). Scale bars: 100 µm in A-E); 30 µm (K-P). The P values were calculated with a two-sample Wilcoxon rank-sum test. GB, gallbladder; BD, bile duct. Brian C. Gibbs et al. Biology Open 2016;bio.015750 © 2016. Published by The Company of Biologists Ltd