Fig. 2. Temporal map of genes clustering with the expression profile of Lmx1a.(A) Heat-map visualization obtained by HCL of genes clustering with Lmx1a.

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Fig. 2. Temporal map of genes clustering with the expression profile of Lmx1a.(A) Heat-map visualization obtained by HCL of genes clustering with Lmx1a (black arrow) in order to identify transcription factors or transcriptional targets that may play an important role in mdDA specification and differentiation. Temporal map of genes clustering with the expression profile of Lmx1a.(A) Heat-map visualization obtained by HCL of genes clustering with Lmx1a (black arrow) in order to identify transcription factors or transcriptional targets that may play an important role in mdDA specification and differentiation. Within this cluster we identified novel transcription factor family members expressed within this region, Onecut2 and Onecut3 (red arrows). (B) The exact expression level over the E10.5-E13.5 window is plotted for Lmx1a, FoxA1, Oncut3, Onecut2, En2 and Neurod1, all present in the heat map presented in (A). Koushik Chakrabarty et al. Biology Open 2012;1:693-704 © 2012. Published by The Company of Biologists Ltd