Clustering of CD14 in the plane of the plasma membrane of LPS-stimulated cells. Clustering of CD14 in the plane of the plasma membrane of LPS-stimulated.

Slides:

Advertisements

Similar presentations

Sec1p requires Boi1/2p for proper localization.

Advertisements

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Volume 91, Issue 1, Pages (January 2017)

The innervation of synovium of human osteoarthritic joints in comparison with normal rat and sheep synovium A. Eitner, J. Pester, S. Nietzsche, G.O.

Figure 2 Binding of the patient's IgG to Purkinje cells is inhibited by pretreatment of rat tissue with anti-VGCC antibodies, confirming specificity of.

Published in Thyroid. January 2014, 24(1): DOI: /thy

Fig. 7. Inhibitory mechanisms of C12G6.

Volume 11, Issue 8, Pages (April 2001)

Alpha-synuclein induces autophagy in microglial cells.

Evaluation of LC3 and ATG13 dynamics in AS-stimulated microglial cells using live-imaging. Evaluation of LC3 and ATG13 dynamics in AS-stimulated microglial.

Fig. 8. CCA and ChQ treatment induce accumulation of F-actin rings.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Fig. 1. Prestin in mouse outer hair cells is localized along the lateral wall of the cell along with β-catenin and Na/K ATPase.Shown are cartoons of the.

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty.

Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. (A,B)

Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.

Table 2. Cell surface abundance of β1 integrin measured by flow cytometry.DDR wild type and DDR over-expressing cells were treated with deoxymannojirimycin.

Fig. 1. E-cadherin localizes in nano-scale clusters.

TWEAK downregulates SIRT3 in cultured tubular cells and this is prevented by crotonate. TWEAK downregulates SIRT3 in cultured tubular cells and this is.

The initial targeting of Sec61b mRNA to the ER is partially dependent on ribosomes and translation. The initial targeting of Sec61b mRNA to the ER is partially.

Don1 localization in vps13Δ cells.

FAK tyrosine phosphorylation is unaffected by loss of LAR activity.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

Loss of LAR phosphatase activity is associated with decreased adhesion complex formation. Loss of LAR phosphatase activity is associated with decreased.

TWEAK increases histone crotonylation in kidney tubular cells.

Reconstitution of GBF1 recruitment to Golgi membranes in a cell-free assay. Reconstitution of GBF1 recruitment to Golgi membranes in a cell-free assay.

Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. Transbilayer distribution of cholesterol in.

Macrophage-produced IL-10 is required but not sufficient for macrophage-stimulated proliferation of ADPKD cells. Macrophage-produced IL-10 is required.

SYCE2 potentiates the steady-state ATM activity.

The SIM domain of RAD51 is required for HR

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

ABCG2-K86M shows a markedly reduced surface expression.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Graf-dependent GEEC endocytosis is required for EGFR internalization and degradation at high Spi. Graf-dependent GEEC endocytosis is required for EGFR.

Impact of LAMP interaction on TAPL function.

Regulation of lipid droplet formation by PI3-kinase activity.

Stimulation of cells with FFAR4 agonist.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

Peroxisome speeds were slower in patient and control cells.

Fig. 2. Acetylation stiffens primary cilia.

The influence of SHIP2 on cell migration in breast cancer cells.

Segments R1, R3, R6 and R7, and GTPase activity of Mfn2ΔTM are necessary for mitochondrial fragmentation in COS7 cells. Segments R1, R3, R6 and R7, and.

Biphasic increase of PI(4,5)P2 level in LPS-stimulated cells.

Association of NM-HA and NM-GFP with SGs is transient.

The number of neurons presenting crosstalk from cAMP to the ERK1/2 cascade increases with age. The number of neurons presenting crosstalk from cAMP to.

PtdIns(4,5)P2 dependence of TbEpsinR and TbCALM membrane targeting.

The expression pattern of marker proteins for sensory neuron subgroups changes during postnatal maturation. The expression pattern of marker proteins for.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Sensory neurons grow and downregulate UCHL1 expression levels during postnatal maturation. Sensory neurons grow and downregulate UCHL1 expression levels.

Electron micrographs of BHK antisense CHC cells incubated with a CTB-HRP conjugate. Electron micrographs of BHK antisense CHC cells incubated with a CTB-HRP.

CD151-knockdown cells show an aberrant spreading response to PMA

BMOL3 LPCs can differentiate towards the hepatocyte lineage.

Vps36 interacts with Smo in the absence of Hh

Ubiquitin mediates the interaction between Smo and Vps36.

Role of Src and PTP-PEST in upregulation of tyrosine phosphorylation of paxillin and FAK. (A) Mock and D11 cells were pre-incubated with PP2 or PP3 (10 µM,

Ezrin phenotypes in the MVID intestine.

Tsg protein stimulates endothelial cell migration, proliferation and collagen gel spheroid sprouting. Tsg protein stimulates endothelial cell migration,

Distinct mitochondrial membrane potential, ATP-to-ADP ratio and NAD(P)H responses to glucose and methylsuccinate. Distinct mitochondrial membrane potential,

Phenotype of S. pombe cells in the presence of B21P2 or LMB

Vps36 regulates the accumulation of Smo and Hh signaling activity.

Effect of myosin Vb shRNA on ezrin phosphorylation and microvilli organization in Caco-2 cells. Effect of myosin Vb shRNA on ezrin phosphorylation and.

Ctip2 directly regulates Notch1 expression in differentiated cells.

Fig. 5 GP38 localization to the viral envelope and plasma membrane.

Number of microparticles (MPs) and distribution of MP surface markers in patients with SLE and HCs. Three aliquots of MPs were incubated with (1) anti-CD3,

Geldanamycin-induced endocytosis and down-regulation of ErbB2 in cells expressing ErbB2 only. Geldanamycin-induced endocytosis and down-regulation of ErbB2.

EGF induces HPSE nucleolar localization in human BMBC cells.

Presentation transcript:

Clustering of CD14 in the plane of the plasma membrane of LPS-stimulated cells. Clustering of CD14 in the plane of the plasma membrane of LPS-stimulated cells. J774 cells were left unstimulated (A,E) or exposed to 100 ng/ml LPS for 5 min (B,F), 30 min (C,G) or 60 min (D). Subsequently, they were incubated with a rat antibody against CD14 followed by goat anti-rat IgG conjugated to 10-nm gold (A–D), or CTX followed by a rabbit antibody against CTX and goat anti-rabbit IgG conjugated to 6-nm gold (E–G) to visualize CD14 and GM1, respectively. After surface labeling, plasma membrane sheets were prepared through mechanical cleavage of cells and examined under an electron microscope. In A–D, CD14 singlets and doublets, and clusters are marked with small and large circles, respectively. Scale bar: 500 nm. (H) Distribution of cluster sizes of gold particles marking CD14 and GM1 before and during stimulation of cells with 100 ng/ml LPS. Data are expressed as the percentage of total gold particles found at a given time point on a 100-μm2 area of the plasma membrane. The mean±s.e.m. of three or four experiments is shown. Agnieszka Płóciennikowska et al. J Cell Sci 2015;128:4096-4111 © 2015. Published by The Company of Biologists Ltd